Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears

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Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.Vector Laboratories’ enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios. Specific covalent linkages are chosen to provide stable, highly active conjugates.These enzyme conjugates are suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications.Horseradish Peroxidase Streptavidin is produced by our own coupling procedure which preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears. In addition, sensitive detection systems for a variety of solid-phase assays utilizing microtitration plates or nitrocellulose have been developed using this conjugate. Horseradish Peroxidase Streptavidin is supplied as a concentrate （1mg/ml） or as a ready-to-use （R.T.U.） stabilized solution （100 ml, 1µg/ml） in a bottle fitted with a drop dispenser top.

CERTIFICATE OF ANALYSISProduct R.T.U HORSERADISH PEROXIDASE STREPTAVIDINCatalog No. SA-5704Amount 100 mlLot No. X1103Concentration 1.0 μg/ml active conjugate*1.5Molar ratio of active conjugateStorage Conditions Refrigerate; DO NOT FREEZE* This product is provided at a ready-to-use concentration in a solution whichpreserves the activity on the conjugate when stored as recommended.For most applications the recommended incubation time is 30 minutes.Do not use sodium azide with this product since it is an inhibitor of horseradishperoxidase.FOR LABORATORY USE.

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