Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor 488, eBioscience™

货号：53-4724-80

规格：25 µg

价格：1188

产品类型：小鼠同型对照

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：eBM2a

荧光染料：Alexa Fluor 488

类型:	同型对照	同型对照：
浓度：	0.5 mg/mL	用法：	Assay-Dependent（Flow）； Assay-Dependent（ICC）; Assay-Dependent（IHC）; Assay-Dependent（IF）; Assay-Dependent（Ctrl）

产品详细信息Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: Alexa Fluor® 488 Mouse IgG2a, K Isotype Control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.Applications Tested: Alexa Fluor® 488 Mouse IgG2a, K Isotype Control has been tested. This can be used at the same concentration as the experimental antibody.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.仅用于科研。不用于诊断过程。

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参考文献：

1. PloS oneDengue immune sera enhance Zika virus infection in human peripheral blood monocytes through Fc gamma receptors."53-4724 was used in Flow cytometry/Cell sorting to examine the mechanisms of antibody dependent enhancement of Zika virus infection in primary human cells."AuthorsLi M,Zhao L,Zhang C,Wang X,Hong W,Sun J,Liu R,Yu L,Wang J,Zhang F,Jin XYear2019 2. Stem cell reportsComparative miRNA-Based Fingerprinting Reveals Biological Differences inHuman Olfactory Mucosa- and Bone-Marrow-Derived Mesenchymal Stromal Cells."53-4724 was used in Flow cytometry/Cell sorting to investigate the biological differences in human olfactory mucosa- and bone-marrow-derived mesenchymal stromal cells."AuthorsLindsay SL,Johnstone SA,McGrath MA,Mallinson D,Barnett SCYear2016 3. PloS oneTLR4 Expression Is Associated with Left Ventricular Dysfunction in Patients Undergoing Coronary Artery Bypass Surgery."53-4724 was used in Flow cytometry/Cell sorting to explore a pathogenic role for TLR4 in ischaemic myocardial dysfunction."AuthorsAvlas O,Bragg A,Fuks A,Nicholson JD,Farkash A,Porat E,Aravot D,Levy-Drummer RS,Cohen C,Shainberg A,Arad M,Hochhauser EYear2016 4. American journal of nephrologyIron sucrose impairs phagocytic function and promotes apoptosis in polymorphonuclear leukocytes."53-4724 was used in Flow cytometry/Cell sorting to elucidate the mechanisms and effects of the use of IV iron preparations against bacteria."AuthorsIchii H,Masuda Y,Hassanzadeh T,Saffarian M,Gollapudi S,Vaziri ND

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