货号：05-220-1B

规格：100ml

价格：2240

产品类型：间充质干细胞培养

品牌：BI

MSCgo™ Chondrogenic Differentiation Medium isa serum-free (SF) and xeno-free (XF) formulation developed for optimal differentiation of human mesenchymal stem cells (hMSC) to mature chondrocytes. The MSCgo™ Chondrogenic Differentiation Medium isvalidated to efficiently differeniate hMSC from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC), and umbilical cord tissue (UC-MSC).

The MSCgo™ Chondrogenic Differentiation Medium kit includes a basal medium and supplement, containing all growth factors and supplements necessary for a complete chondrogenic differentiation medium. No additional supplements are required.

The MSCgo™ Chondrogenic Differentiation protocol is part of a complete system for multipotency evaluation of hMSCs. This kit enables reliable differentiation of hMSCs to mature chondrocytes without background differentiation or interruption in cellular metabolism.

- Serum-free, xeno-free medium

- All required growth factors and supplements included in kit

- Reliable differentiation to mature chondrocytes

- Each lot is application tested

- Does not contain antibiotics

- MSCgo Chondrogenic Differentiation Basal Medium: 100 mL

- MSCgo Chondrogenic Differentiation Supplement Mix : 10 mL

Chondrogenic differentiation of hMSC in 3D spheroid culture in the formation of cartilage with a typical extracellular matrix rich of Aggrecan. Aggrecan is a proteoglycan that can be used as an indicator for cartilage formation and can be detected with Alcian Blue, a dark-blue copper-containing dye that acts as an indication of mature chondrocytes. The staining intensity can be vary using different hMSC (e.g. source, age, and passage number).

hMSC-AT hMSC-BM hMSC-CT

Cartilage differentiation results of hMSC from various sources after 21 day assay using MSCgo™ Chondrogenic followed by Alcian Blue staining.

hMSC-AT hMSC-BM hMSC-CT

Histological images of mature chondrocytes surrounded by a cartilage matrix after a 21-day differentiation assay using MSCgo™ Chondrogenic Medium followed by Toluidine blue (40x).