CD152 (CTLA-4) Monoclonal Antibody (UC10-4B9), PE, eBioscience™

货号：12-1522-82,12-1522-83,12-1522-81

规格：100 µg,200 µg,50 µg

价格：2028,3198,1065

产品类型：流式抗体

品牌：eBioscience

抗原：CD152

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：UC10-4B9

荧光染料：PE

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™
浓度：	0.2 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The UC10-4B9 monoclonal antibody reacts with mouse CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells at low level and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated stateby inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in a large proportion of newly synthesized CTLA-4 in response to TCR signaling to be localized intracellularly.Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.Applications Reported: This UC10-4B9 antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis.Applications Tested: This UC10-4B9 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the FoxP3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.仅用于科研。不用于诊断过程。

数据

CD152 (CTLA-4) Antibody (12-1522-83) in FlowC57Bl/6 splenocytes were unstimulated (left) or stimulated with Con A for 3 days (right). Cells were intracellularly stained with Anti-Mouse CD4 eFluor® 450 (Product # 48-0041-82) and 0.125 µg of Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (blue histogram) or 0.125 µg of Anti-Mouse CD152 (CTLA-4) PE (purple histogram) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the CD4+ gate were used for analysis.

推荐产品：

-Intracellular Fixation & Permeabilization Buffer Set（货号#88-8824）-Foxp3 / Transcription Factor Staining Buffer Set（货号#00-5523-00）-Flow Cytometry Staining Buffer（货号#00-4222-57/26）

参考文献：

1. Journal of immunology (Baltimore, Md. : 1950)Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency."12-1522 was used in Flow cytometry/Cell sorting to suggest persistent viral lytic gene expression during latency causes chronic inflammatory response inducing exhaustion of resident T cells in the ependyma."AuthorsMenendez CM,Jinkins JK,Carr DJYear2016SpeciesMouse 2. PLoS pathogensA Human Trypanosome Suppresses CD8+ T Cell Priming by Dendritic Cells through the Induction of Immune Regulatory CD4+ Foxp3+ T Cells."12-1522 was used in Flow cytometry/Cell sorting to demonstrate a newly described mechanism of CD8+ T cell priming by CD4+ Foxp3+ T cells during Trypanosoma cruzi infection."AuthorsErsching J,Basso AS,Kalich VL,Bortoluci KR,Rodrigues MMYear2016SpeciesMouse 3. OncotargetAmphiregulin activates regulatory T lymphocytes and suppresses CD8+ T cell-mediated anti-tumor response in hepatocellular carcinoma cells."12-1522 was used in Flow cytometry/Cell sorting to investigate how hepatocellular carcinoma cells alter endogenous anti-tumor immunity and their related signaling pathways."AuthorsYuan CH,Sun XM,Zhu CL,Liu SP,Wu L,Chen H,Feng MH,Wu K,Wang FBYear2015SpeciesMouse 4. EMBO molecular medicineADAP and SKAP55 deficiency suppresses PD-1 expression in CD8+ cytotoxic T lymphocytes for enhanced anti-tumor immunotherapy."12-1522 was used in Flow cytometry/Cell sorting to investigate the effects of the ADAP-SKAP55 signaling module on cytotoxicity, PD-1 expression, and anti-tumour immunity."AuthorsLi C,Li W,Xiao J,Jiao S,Teng F,Xue S,Zhang C,Sheng C,Leng Q,Rudd CE,Wei B,Wang HYear2015SpeciesMouse 5. Shock (Augusta, Ga.)Programmed cell death receptor ligand 1 modulates the regulatory T cells' capacity to repress shock/sepsis-induced indirect acute lung injury by recruiting phosphatase SRC homology region 2 domain-containing phosphatase 1."12-1522 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which the programmed cell death receptor 1 ligand suppresses lung injury."AuthorsTang L,Bai J,Chung CS,Lomas-Neira J,Chen Y,Huang X,Ayala A

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