货号：119307

规格：50µg

价格：1260

产品类型：流式抗体

品牌：Biolegend

物种：小鼠

宿主：仓鼠

抗体亚型：IgG

荧光染料：PE

Description

CD34 is a highly glycosylated hematopoietic progenitor antigen. Two isoforms of CD34 have been reported to be generated by alternative splicing. This antigen is expressed on hematopoietic progenitors as well as on endothelial cells, brain, and testis. CD34 is thought to function as an adhesion molecule for early hematopoietic progenitors mediating the attachment of stem cells to extracellular matrix or stromal cells. CD34 is phosphorylated on serine residues by PKC.

• Isotype Control

• PE Rat IgG2a, κ Isotype Ctrl

• Reactivity

• Mouse

• Immunogen

• Cells transfected with mouse CD34

• Formulation

• Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

• Preparation

• The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

• Concentration

• 0.2 mg/ml

• Storage & Handling

• The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.

• Application

• FC - Quality tested

• Recommended Usage

• Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

• Excitation Laser

• Blue Laser (488 nm)

• Application Notes

• The MEC14.7 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting6, and immunohistochemistry of acetone-fixed frozen sections and paraffin-embedded sections2,4,5,6.

• Application References(PubMed link indicates BioLegend citation)

• 1. Garlanda C,et al.1997.Eur. J. Cell Biol.73:368. (FC)2. Knowles HJ,et al.2004.Circ. Res.95:162. (IHC)3. Trempus CS,et al.2003.J. Invest. Dermatol.120:501.4. Winding B,et al.2002.Clin. Cancer Res.8:1932. (IHC)5. Voswinckel R,et al.2003.Circ. Res.93:372. (IHC)6. Kairaitis LK,et al.2005.Am. J. Physiol. Renal. Physiol.288:F198. (IHC, WB)7. Ao A,et al.2008.P. Natl. Acad. Sci. USA105:7821.PubMed