CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.
Isotype Control
APC/Cy7 Rat IgG2b, κ Isotype Ctrl
Reactivity
Mouse, Human,Cross-Reactivity:Baboon, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey, Horse (Equine), Cattle (Bovine, Cow), Swine (Pig, Porcine), Dog (Canine), Cat (Feline)
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Application
FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Red Laser (633 nm)
Application Notes
Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18that is located between amino acids 145 and 18620. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, andin vivoinhibition of DTH4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103014). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 103046) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation)
1. Trowbridge IS,et al.1982.Immunogenetics15:299. (ICFC, IP, CMCD)2. Katoh S,et al.1994.J. Immunol.153:3440. (ELISA)3. Budd RC,et al.1987.J. Immunol.138:3120. (IP)4. Camp RL,et al.1993.J. Exp. Med.178:497. (Block)5. Weiss JM,et al.1997.J. Cell Biol.137:1137. (Block)6. Frank NY,et al.2005.Cancer Res.65:4320. (IHC)PubMed7. Cuff CA,et al.2001.J. Clin. Invest.108:1031. (IHC)8. Lee JW,et al.2006.Nature Immunol.8:181.9. Zhang N,et al.2005.J. Immunol.174:6967.PubMed10. Huabiao C,et al.2005.J. Immunol.175:591.PubMed11. Gui J,et al.2007.Int. Immunol.19:1201.PubMed12. Wang XY,et al.2008.Blood111:2436.PubMed13. Kenna TJ,et al.2008.Blood111:2091.PubMed14. Yamazaki J,et al.2009.BloodPubMed15. Kmieciak M,et al.2009.J. Transl. Med.7:89. (FC)PubMed16. Chen YW,et al.2010.Mol. Cancer Ther.9:2879.PubMed17. Zheng Z,et al.1995.J. Cell. Biol.130:485.18. Wiranowska M,et al.2010.Int. J. Cancer127:532.19. Hirokawa Y,et al.2014.Am J Physiol Gastrointerest Liver Physiol.306:547.PubMed20. Sandmaier BM,et al.1998.Blood91:3494.21. Nagaoka M,et al.2014.J Immunol.193:2812.PubMed22. Young KH,et al.2014.Cancer Immunol Res.2:1011.PubMed
QQ:1749072012