| The MB19-1 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. Staining of B cells with MB19-1 and its conjugates is usually dimmer than the rat anti-mouse CD19 antibody, clone 6D5.Applications Reported: The MB19-1 antibody has been reported for use in flow cytometric analysis.Applications Tested: The MB19-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 1. PloS one Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential."17-0191 was used in Flow cytometry/Cell sorting to find markers associated with non-conventional T cell subsets, including γδ T cells and invariant natural killer T cells."Authors Qualai J,Li LX,Cantero J,Tarrats A,Fernández MA,Sumoy L,Rodolosse A,McSorley SJ,Genescà M2. Protein and cell G-CSF is a key modulator of MDSC and could be a potential therapeutic target in colitis-associated colorectal cancers."17-0191 was used in Flow cytometry/Cell sorting to indicate that G-CSF is a critical regulating molecule in the migration, proliferation and functional maintenance of myeloid derived suppressor cells."Authors Li W,Zhang X,Chen Y,Xie Y,Liu J,Feng Q,Wang Y,Yuan W,Ma J3. Journal of virology Pentosan Polysulfate: a Novel Glycosaminoglycan-Like Molecule for Effective Treatment of Alphavirus-Induced Cartilage Destruction and Inflammatory Disease."17-0191 was used in Flow cytometry/Cell sorting to demonstrate the potential of glycotherapeutics as a new class of treatment for infectious arthritis."Authors Herrero LJ,Foo SS,Sheng KC,Chen W,Forwood MR,Bucala R,Mahalingam S4. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsDepletion of foxp3(+) T cells abrogates tolerance of skin and heart allografts in murine mixed chimeras without the loss of mixed chimerism."17-0191 was used in Flow cytometry/Cell sorting to investigate the relative contribution of central and peripheral mechanisms to the generation of skin and heart allograft tolerance in murine mixed chimeras."Authors Shinoda K,Akiyoshi T,Chase CM,Farkash EA,Ndishabandi DK,Raczek CM,Sebastian DP,Pelle PD,Russell PS,Madsen JC,Colvin RB,Alessandrini A5. PloS one Methotrexate treatment causes early onset of disease in a mouse model of Ross River virus-induced inflammatory disease through increased monocyte production."17-0191 was used in Flow cytometry/Cell sorting to study infiltration of inflammatory cells to skeletal muscle in Ross River virus disease in response to MTX treatment."Authors Taylor A,Sheng KC,Herrero LJ,Chen W,Rulli NE,Mahalingam S |
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