| Description: The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 1. Journal of virology Mice Expressing Minimally Humanized CD81 and Occludin Genes Support Hepatitis C Virus Uptake In Vivo. "45019382 was used in flow cytometry to study hepatitis C virus infection in mice with humanized CD81 and occludin" Authors Ding Q,von Schaewen M,Hrebikova G,Heller B,Sandmann L,Plaas M,Ploss A2.Oncoimmunology CXCL16-positive dendritic cells enhance invariant natural killer T cell-dependent IFNγ production and tumor control. "45-0193 was used in Flow cytometry/Cell sorting to study the co-stimulatory role of CXCR6/CXCL16 interactions in glycolipid-dependent invariant natural killer T (iNKT) cell activation and tumour control, showing that CXCL16-positive dendritic cells enhance iNKT cell-dependent IFNγ production and tumor control." Authors Veinotte L,Gebremeskel S,Johnston B3. JCI insight A small-molecule inhibitor of SHIP1 reverses age- and diet-associated obesity and metabolic syndrome. "45-0193 was used in Flow cytometry/Cell sorting to investigate how SHIPi represents an approach that permits control of obesity and diet-induced metabolic syndrome without apparent toxicity." Authors Srivastava N,Iyer S,Sudan R,Youngs C,Engelman RW,Howard KT,Russo CM,Chisholm JD,Kerr WG4. Cell The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. "45-0193 was used in Flow cytometry/Cell sorting to study how OTULIN may be critical in restraining life-threatening spontaneous inflammation and maintaining immune homeostasis." Authors Damgaard RB,Walker JA,Marco-Casanova P,Morgan NV,Titheradge HL,Elliott PR,McHale D,Maher ER,McKenzie ANJ,Komander D5. Cancer discovery IL35-Producing B Cells Promote the Development of Pancreatic Neoplasia. "45-0193 was used in Flow cytometry/Cell sorting to identify a previously unrecognised role for IL35-producing CD1d(hi)CD5(+) B cells in the pathogenesis of pancreatic cancer." Authors Pylayeva-Gupta Y,Das S,Handler JS,Hajdu CH,Coffre M,Koralov SB,Bar-Sagi D |
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