anti-mouse CD19 (eBio1D3) FG 功能抗体

2024-10-16

anti-mouse CD19 (eBio1D3) FG 功能抗体

货号:16-0193-81,16-0193-85

规格:50 μg,500 μg

价格:1081,3911

产品类型:流式抗体

品牌:eBioscience

抗原:CD19

物种:小鼠

宿主:大鼠

抗体亚型:IgG

克隆号:eBio1D3 (1D3)

荧光染料:Fg Purified

抗体类型:单抗同型对照:IgG2a, kappa
免疫原性:应用领域:Flow;MISC;FN;IHC
推荐用法:0.5 µg/test(flow)

Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/μg antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
数据

Functional Grade Purified (Product # 16-4321-82) (open histogram) or 0.25 µg of Anti-Mouse CD19 Functional Grade Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87).Cells in the lymphocyte gate were used for analysis.

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参考文献:
1. PloS oneCRISPR-Mediated Triple Knockout of SLAMF1, SLAMF5 and SLAMF6 Supports Positive Signaling Roles in NKT Cell Development."16-0193 was used in Flow cytometry/Cell sorting to elucidate the contribution of the SLAM receptors in immunodysregulation observed in X-linked lymphoproliferative syndrome Type 1."Authors Huang B,Gomez-Rodriguez J,Preite S,Garrett LJ,Harper UL,Schwartzberg PL2.WASH maintains NKp46+ ILC3 cells by promoting AHR expression."16-0193 was used in Flow cytometry/Cell sorting to study how WASH-mediated AHR expression has a critical function in the maintenance of NKp46(+) ILC3s."Authors Xia P,Liu J,Wang S,Ye B,Du Y,Xiong Z,Han ZG,Tong L,Fan Z3.The EMBO journalMolecular requirements of the B-cell antigen receptor for sensing monovalent antigens."16-0193 was used in Flow cytometry/Cell sorting to study the effects of differing monovalent stimuli on B-cell antigen receptor signalling and organisation."Authors Volkmann C,Brings N,Becker M,Hobeika E,Yang J,Reth M4. Nature immunology Arginase 1 is an innate lymphoid-cell-intrinsic metabolic checkpoint controlling type 2 inflammation."16-0193 was used in Flow cytometry/Cell sorting to identify Arg1 as a key regulator of group 2 innate lymphoid cell bioenergetics that control proliferative capacity and pro-inflammatory functions."Authors Monticelli LA,Buck MD,Flamar AL,Saenz SA,Tait Wojno ED,Yudanin NA,Osborne LC,Hepworth MR,Tran SV,Rodewald HR,Shah H,Cross JR,Diamond JM,Cantu E,Christie JD,Pearce EL,Artis D5.Journal of immunology (Baltimore, Md. : 1950) Survival of Igα-Deficient Mature B Cells Requires BAFF-R Function."16-0193 was used in Flow cytometry/Cell sorting to assess the importance of the expression of a functional B-cell receptor for B cell maintenance."Authors Levit-Zerdoun E,Becker M,Pohlmeyer R,Wilhelm I,Maity PC,Rajewsky K,Reth M,Hobeika E

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