View (160) other CD45 antibodies Invitrogen CD45 Monoclonal Antibody (HI30), FITC, eBioscience™

货号：11-0459-42,11-0459-41

规格：100tests,25tests

价格：1873,831

产品类型：流式抗体

品牌：eBioscience

抗原：CD45

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：HI30

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test（flow）

产品详细信息Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: The HI30 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

数据

CD45 Antibody (11-0459-42) in Flow Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD45 FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.PloS oneGuanine-Rich Sequences Are a Dominant Feature of Exosomal microRNAs across the Mammalian Species and Cell Types."11-0459 was used in Flow cytometry/Cell sorting to statistically analyse miRNA microarray data, revealing that guanine-rich sequences are a dominant feature of exosome-dominant miRNAs."AuthorsMomose F,Seo N,Akahori Y,Sawada S,Harada N,Ogura T,Akiyoshi K,Shiku H2.Stem cell research and therapyHuman umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism."11-0459 was used in Flow cytometry/Cell sorting to investigate the potential of hUCMSCs in improving ovarian function and withstanding ovarian senescence."AuthorsLi J,Mao Q,He J,She H,Zhang Z,Yin C3.Stem cell reportsRetinoic acid regulates hematopoietic development from human pluripotent stem cells."11-0459 was used in Flow cytometry/Cell sorting to evaluate the effects of retinoic acid signalling on the development of blood and on generated haematopoietic progenitors."AuthorsRönn RE,Guibentif C,Moraghebi R,Chaves P,Saxena S,Garcia B,Woods NB4.Clinical cancer research : an official journal of the American Association for Cancer ResearchGliomas promote immunosuppression through induction of B7-H1 expression in tumor-associated macrophages."11-0459 was used in Flow cytometry/Cell sorting to demonstrate that gliomas can upregulate B7-H1 expression in circulating monocytes and tumor-infiltrative macrophages through modulation of autocrine/paracrine IL-10 signaling."AuthorsBloch O,Crane CA,Kaur R,Safaee M,Rutkowski MJ,Parsa AT5.PloS oneIdentification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells."11-0459 was used in Flow cytometry/Cell sorting to provide evidence of heterogeneity among bone marrow stromal cells and identify potential candidate proteins for quantifying MSCs in vitro."AuthorsRosu-Myles M,She YM,Fair J,Muradia G,Mehic J,Menendez P,Prasad SS,Cyr TD

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