Cell Stimulation Cocktail (plus protein transport inhibitors 细胞刺激剂（PMA、 离子霉素）（含BFA & 莫能菌素）(500X)/广谱激活剂

货号：00-4975-03,00-4975-93

规格：4 x 100 µL,100 µL

价格：3527,1172

产品类型：免疫细胞培养

品牌：eBioscience

Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) 是佛波醇酯 12-十四酸酯 13-乙酸酯 (PMA)、离子霉素、Brefeldin A 和莫能菌素的混合物。使用 PMA 和离子霉素进行处理足以诱导大多数细胞类型激活，产生细胞因子。Brefeldin A 和莫能菌素可导致内质网和高尔基体中出现分泌性蛋白蓄积。因此，Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) 可用于细胞因子和其他分泌性蛋白的诱导及随后的细胞内检测，适用于体外培养和来自体内的细胞。对于未受刺激的溶媒对照，请参见 Protein Transport Inhibitor Cocktail (500X)（货号#00-4980）。

数据：

图1 Data for Cell Stimulation Cocktail (plus protein transport inhibitors) (500X)Mouse splenocytes were cultured with 1X Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) for 16 hours. Cultures were harvested, fixed and permeabilized with IC Fixation Buffer (cat. 00-8222) and Permeabilization Buffer (cat. 00-8333). The cells were then stained with Anti-Mouse CD4 FITC (cat. 11-0042), Anti-Mouse IFN gamma PE (12-7311) and Anti-Mouse TNF alpha APC (cat. 17-7321). Cells in the lymphocyte gate were used for analysis.

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▪免疫细胞培养基▪免疫细胞培养刺激剂 ▪流抗抗体

技术参数

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