Pierce Protease and Phosphatase Inhibitor Mini Tablets,EDTA-free蛋白酶和磷酸化酶混合抑制片剂，不含EDTA-Bio-Rad-Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱

Pierce Protease and Phosphatase Inhibitor Mini Tablets,EDTA-free蛋白酶和磷酸化酶混合抑制片剂，不含EDTA

2024-10-16

Pierce Protease and Phosphatase Inhibitor Mini Tablets,EDTA-free蛋白酶和磷酸化酶混合抑制片剂，不含EDTA

货号：A32961

规格：20 tablets

价格：3120

产品类型：蛋白提取/纯化

品牌：Thermo Fisher

Thermo Scientific Pierce 蛋白酶和磷酸酶抑制剂微型片剂（无 EDTA）同时含有蛋白酶抑制剂和磷酸酶抑制剂，以提供广谱完全保护，防止发生去磷酸化和蛋白水解降解。蛋白酶和磷酸酶抑制剂微型片剂（无 EDTA）的特点：-改进的配方—片剂快速溶解形成澄清溶液-经济实惠—相同体积的情况下，比其他市售配方更节约成本-便利—即用型、完全披露、广谱配方-兼容—直接与 Pierce BCA 测定配合使用-无 EDTA—配方不含 EDTA，金属蛋白酶抑制剂（仅适用于无 EDTA 配方）Pierce 蛋白酶和磷酸酶抑制剂微型片剂含有抑肽酶、苯丁抑制素、E-64、EDTA 和亮肽素，以防止丝氨酸蛋白酶、半胱氨酸蛋白酶和天冬氨酸蛋白酶发生降解。为防止丝氨酸/苏氨酸和酪氨酸磷酸酶的去磷酸化活性，每片还含有氟化钠、原钒酸钠、焦磷酸钠和 β-磷酸甘油。Pierce 蛋白酶和磷酸酶抑制剂片剂在 4°C 下可保持稳定 12 个月，每片足以溶解为 10 mL 溶液。Pierce 蛋白酶和磷酸酶抑制剂微型片剂是领先的全面抑制剂片剂。在蛋白提取和蛋白纯化过程中，向 10 mL 裂解缓冲液中添加一片可完全保护蛋白酶和磷酸酶活性。该抑制剂有两种配方，其中一种含有 EDTA、一种不含 EDTA。虽然两种配方均含有金属蛋白酶抑制剂，但无 EDTA 配方可用于蛋白活性需要二价阳离子的应用。同样，EDTA 和某些磷酸酶抑制剂会干扰固定化金属螯合亲和层析 (IMAC) 和 2D 电泳。在执行这些程序之前，对样品进行透析或脱盐。由于不含 AEBSF，蛋白酶和磷酸酶抑制剂配方与质谱法兼容。

优点：

-改进的配方—片剂快速溶解形成澄清溶液-经济实惠—相同体积的情况下，比其他市售配方更节约成本-便利—即用型、完全披露、广谱配方-兼容—直接与 Pierce BCA 测定配合使用-无 EDTA—配方不含 EDTA，金属蛋白酶抑制剂（仅适用于无 EDTA 配方）

数据：

图 1Comparison of protease and phosphatase inhibitor activity of existing versus new Pierce Protease and Phosphatase Inhibitor Mini TabletsProtease Inhibition: Pancreatic extract (100 µL; 0.5 µg/µL) was incubated with fluorescent protease-cleavable substrates for trypsin, cysteine, and metalloprotease/cathepsins in the presence of commercially-available protease inhibitors with and without EDTA. Reactions were incubated for 1 hour at 37ºC and fluorescence was measured. The percent protease inhibition is shown. Phosphatase Inhibition: Kidney extract (25 µL; 0.5 µg/µL) was incubated with a fluorogenic substrate that measures phosphatase activity upon de-phosphorylation. Reactions were incubated for 1 hour at 37ºC and fluorescence was measured. The percent phosphatase inhibition is shown.

图2 Pierce Phosphatase Inhibitor Mini Tablets preserve tyrosine phosphorylationHCT116 cells were serum starved, treated with either EGF for 15 min, or left as control cells. Cell lysates were prepared in IP Lysis Buffer with the current and new Pierce Protease and Phosphatase Inhibitor Tablets (EDTA-free). Lysate (500 µg) was then incubated with 5 µg of phospho-tyrosine antibody overnight at 4ºC. The complex was then incubated with Protein A/G magnetic beads for 1 hr at RT. Beads were washed and low pH elution was performed. The membrane was then probed with EGFR antibody for western blot detection.88669:current protease and phosphatase inhibitor tablets, EDTA-free.A32961:new protease and phosphatase inhibitor tablets, EDTA-free.

Pierce Protease and Phosphatase Inhibitor Mini Tablets,EDTA-free蛋白酶和磷酸化酶混合抑制片剂，不含EDTA

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