ANTI-HUMAN CD45 (HI30) EFLUOR 450
货号：48-0459-41,48-0459-42
规格：25T,100T
价格：1151,2603
产品类型：流式抗体
品牌：eBioscience
抗原：CD45
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：HI30
荧光染料：eFluor 450
| 抗体类型: | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 450 |
| 免疫原性： | | 用法： | 5uL（0.5µg）/test(Flow） |
| Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
CD45 Antibody (48-0459-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (blue histogram) or Anti-Human CD45 eFluor® 450 (purple histogram). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.PloS oneEngineering HIV-1-resistant T-cells from short-hairpin RNA-expressing hematopoietic stem/progenitor cells in humanized BLT mice.2013"48-0459 was used in Flow cytometry/Cell sorting to investigate combination approaches against HIV, showing that short-hairpin RNA-expressing hematopoietic stem/progenitor cells can produce HIV-1-resistant T cells."Authors Ringpis GE,Shimizu S,Arokium H,Camba-Colón J,Carroll MV,Cortado R,Xie Y,Kim PY,Sahakyan A,Lowe EL,Narukawa M,Kandarian FN,Burke BP,Symonds GP,An DS,Chen IS,Kamata M |
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