ANTI-HU CD105 (ENDOGLIN)(SN6) EFLUOR 450
货号:48-1057-41,48-1057-42
规格:25T,100T
价格:1893,2573
产品类型:流式抗体
品牌:eBioscience
抗原:CD105
物种:人
宿主:小鼠
抗体亚型:IgG
克隆号:SN6
荧光染料:eFluor 450
| 抗体类型: | 单抗 | 同型对照: | IgG1, kappa |
| 免疫原性: | | 用法: | 5uL(0.25µg)/test(Flow) |
| Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin. CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.Applications Reported: This SN6 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SN6 antibody has been pre-titrated and tested by flow cytometric analysis of the U937 cell line. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
CD105 (Endoglin) Antibody (48-1057-41) in FlowStaining of U937 cells with Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (blue histogram) or Anti-Human CD105 (Endoglin) eFluor® 450 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 780, were used for analysis. |
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| 参考文献: |
| 1.Developmental biologyBMP signaling mediated by constitutively active Activin type 1 receptor (ACVR1) results in ectopic bone formation localized to distal extremity joints.2015"48-1057 was used in Flow cytometry/Cell sorting to characterise a novel mouse model of heterotopic ossification."Authors Agarwal S,Loder SJ,Brownley C,Eboda O,Peterson JR,Hayano S,Wu B,Zhao B,Kaartinen V,Wong VC,Mishina Y,Levi B |