ANTI-HU CD105 SN6 SB645-Bio-Rad-Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱

ANTI-HU CD105 SN6 SB645

2024-10-17

ANTI-HU CD105 SN6 SB645

货号：64-1057-41,64-1057-42

规格：25T,100T

价格：0,3244

产品类型：流式抗体

品牌：eBioscience

抗原：CD105

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号： SN6

荧光染料：Super Bright 645

抗体类型:	单抗	同型对照：	IgG1, kappa
免疫原性：		用法：	5uL（0.06 µg）/test(Flow）

Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin. CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.Applications Reported: The SN6 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SN6 antibody has been pre-titrated and tested by flow cytometric analysis of U937 cell line. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product # SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser

数据

CD105 (Endoglin) Antibody (64-1057-41) in FlowStaining of U937 cells with Mouse IgG1 K Isotype Control Super Bright 645 (Product # 64-4714-82) (blue histogram) or CD105 (Endoglin) Monoclonal Antibody, Super Bright 645 (purple histogram). Total viable cells were used for analysis.

推荐产品：

间充干细胞MSC分选间充干细胞MSC扩增、分化培养基CD105流式抗体细胞因子

技术参数

产品应用 Flow

细胞类型 MSC

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://www.biorod.cn/thread-16716.htm

产品询价需求提交

ANTI-HU CD105 SN6 SB645

相关推荐