ANTI-HUMAN CD34 (4H11) APC-eFluor 780
货号：47-0349-41,47-0349-42
规格：25TESTS,100TESTS
价格：1519,3531
产品类型：流式抗体
品牌：eBioscience
抗原：CD34
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：4H11
荧光染料：APC-eFluor 780
| 抗体类型: | 单抗 | 同型对照： | IgG1, kappa |
| 免疫原性： | | 用法： | 5uL（0.5µg）/test(Flow） |
| Description: The 4H11 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family which also includes endoglycan and podocalyxin. Members of this family are single pass transmembrane proteins with a heavily glycosylated extracellular and N-terminal mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors, and has since been extensively used as a marker to isolate cells capable of hematopoietic cell engraftment. In spite of this, the function of CD34 remains unresolved. In addition to expression on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells, tumor cell lines, and by vascular endothelia in the adult. Epitopes of CD34 have been assigned to three classes (class I, II or III) based on their differential sensitivity to enzymatic cleavage by neuraminidase, chymopapain, or O-glycoprotease. According to this analysis, the 4H11 antibody belongs to class III, indicating that it reacts with a protein epitope.Applications Reported: This 4H11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 4H11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.APC-eFluor® emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
CD34 Antibody (47-0349-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD45 FITC (Product # 11-0459-42) and Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (left) or Anti-Human CD34 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.AgingA novel autosomal recessive TERT T1129P mutation in a dyskeratosis congenita family leads to cellular senescence and loss of CD34+ hematopoietic stem cells not reversible by mTOR-inhibition."47-0349 was used in Flow cytometry/Cell sorting to outline limitations of a potential dyskeratosis congenita therapy based on rapamycin."Authors Stockklausner C,Raffel S,Klermund J,Bandapalli OR,Beier F,Brümmendorf TH,Bürger F,Sauer SW,Hoffmann GF,Lorenz H,Tagliaferri L,Nowak D,Hofmann WK,Buergermeister R,Kerber C,Rausch T,Korbel JO,Luke B,Trumpp A,Kulozik AE2.NeoplasmaGrowth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines.Authors Elknerová K,Lacinová Z,Soucek J,Marinov I,Stöckbauer P |
QQ:1749072012