DYKDDDDK Tag Monoclonal Antibody (FG4R) 标签抗体
货号：MA1-91878,MA1-91878-1MG
规格：100 μg,1 mg
价格：2546,13782
产品类型：标签抗体
品牌：Thermo Fisher
物种：其它
宿主：小鼠
抗体亚型：IgG2b, kappa
克隆号：FG4R
荧光染料：其它
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| 抗体类型: | 单抗 | 同型对照： | lgG2b |
| 免疫原性： | Synthetic peptide(DYKDDDDK)coupled to KLH | 用法： | 1:200-1:500(ICC);1:200-1:500(IF); 3 µg(IP);1:500-1:1000(WB) |
| Product Specific InformationSimilar epitope as the Flag tag from SigmaMA1-91878 detects Flag tag from various samplesMA1-91878 has been successfully used in immunoprecipitation, Western blot and immunofluorescence applications.The MA1-91878 immunogen is synthetic peptide (DYKDDDDK) coupled to KLH.MA1-91878 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.Background/Target InformationDYKDDDDK Tag (FLAG-tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization. |
| 数据 |
DYKDDDDK Tag Antibody (MA1-91878) in IFImmunofluorescent analysis of Chlamydia trachomatis FLAG-tagged IncA (blue) in HeLa cells. Cells were infected with a GFP-expressing strain of Chlamydia trachomatis containing FLAG-IncA under the control of the Tetracycline (Tet) promoter, and either left untreated (0ng/ml Tet) or treated with anhydrotetracycline (10ng/ml Tet). Cells were fixed with 2% paraformaldehyde in PBS for 30 minutes at room temperature, permeabilized and blocked in 10% goat serum, 0.1% BSA, 0.1% saponin in PBS pH 7.4 for 30 minutes at room temperature, and then probed with a FLAG Epitope Tag (DYKDDDDK) monoclonal antibody (Product # MA1-91878) at a dilution of 1:500 for 1 hour at room temperature. Cells were washed with PBS, and incubated with an AlexaFluor 647-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:2000 for 1 hour at room temperature. Images were taken on a fluorescent microscope using a 60X oil-immersion objective. Scale bars represent 20um. Data courtesy of the Innovators Program. |
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| 参考文献： |
| 1.Plant and cell physiologyProtein-protein interactions among xyloglucan-synthesizing enzymes and formation of Golgi-localized multiprotein complexes.2017.Authors Chou YH,Pogorelko G,Young ZT,Zabotina OAMolecular and cellular endocrinologyProtein Phosphatase 1α enhances renal aldosterone signaling via mineralocorticoid receptor stabilization.2017.Authors Nagarajan S,Vohra T,Loffing J,Faresse N2.Molecular neurobiologyThe Ubiquitin Receptor ADRM1 Modulates HAP40-Induced Proteasome Activity.2017.Authors Huang ZN,Her LS3.Development (Cambridge, England)neural tube formation.2017.Authors Balashova OA,Visina O,Borodinsky LN4.Plant and cell physiologyProtein-protein interactions among xyloglucan-synthesizing enzymes and formation of Golgi-localized multiprotein complexes.2017.Authors Chou YH,Pogorelko G,Young ZT,Zabotina OA5.Development (Cambridge, England)The non-canonical BMP and Wnt/β-catenin signaling pathways orchestrate early tooth development.2015.Authors Yuan G,Yang G,Zheng Y,Zhu X,Chen Z,Zhang Z,Chen Y |
产品应用 WB;IF;IP;IP; ICC
QQ:1749072012