SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), eFluor 660, eBioscience™

货号：50-8813-41,50-8813-42

规格：25 Tests,100 Tests

价格：1177,2900

产品类型：流式抗体

品牌：eBioscience

抗原：SSEA1

物种：人/小鼠

宿主：小鼠

抗体亚型：IgM

克隆号：eBioMC-480 (MC-480)

荧光染料：eFluor 660

抗体类型:	单抗	同型对照：	IgM
免疫原性：		用法：	5uL（0.06 µg）/test(Flow）

Description: The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine and human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. Expression of the carbohydrate moiety is also found on mature human granulocytes (on CD15) and some monocytes. SSEA-1 is associated with cell adhesion, migration and differentiation.Applications Reported: This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioMC-480 (MC-480) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of the F9 cell line. This can be used at 5 µL (0.06 µg µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin and acutes disease.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1.The Journal of cell biologyNoncanonical function of DGCR8 controls mESC exit from pluripotency."50-8813 was used in Flow cytometry/Cell sorting to reveal a new noncanonical function of DGCR8 in the modulation of the alternative splicing of Tcf7l1 mRNA in addition to its established function in microRNA biogenesis."Authors Cirera-Salinas D,Yu J,Bodak M,Ngondo RP,Herbert KM,Ciaudo C2.Molecular cellNot All H3K4 Methylations Are Created Equal: Mll2/COMPASS Dependency in Primordial Germ Cell Specification."50-8813 was used in Flow cytometry/Cell sorting to analyse the contribution of MLL2's methyltransferase and CXXC domain in H3K4 trimethylation in mouse embryonic stem cells."Authors Hu D,Gao X,Cao K,Morgan MA,Mas G,Smith ER,Volk AG,Bartom ET,Crispino JD,Di Croce L,Shilatifard A3.NatureNANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers."50-8813 was used in Flow cytometry/Cell sorting to investigate the role of nanog in unipotent primordial germ cells in mice."Authors Murakami K,Günesdogan U,Zylicz JJ,Tang WWC,Sengupta R,Kobayashi T,Kim S,Butler R,Dietmann S,Surani MA4.NatureParent stem cells can serve as niches for their daughter cells.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer5.PLoS geneticsCell reprogramming requires silencing of a core subset of polycomb targets.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer

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