CD140a (PDGFRA) Monoclonal Antibody (APA5), eBioscience™

货号：14-1401-81,14-1401-82

规格：50 µg ,100 µg

价格：717,1196

产品类型：流式抗体

品牌：eBioscience

抗原：CD140a (PDGFRA)

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：APA5

荧光染料：其它

抗体类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：		用法：	1 µg/test(Flow）

Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.Applications Reported: The APA5 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking of ligand binding. (Please use Functional Grade purified APA5 in functional assays.).Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of the NIH/3T3 cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationPDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD140a (PDGFRA) Antibody (14-1401-81) in FlowStaining of NIH/3T3 cells with 0.5 µg of Rat IgG2a K Isotype Control Purified(Product # 14-4321-82) (open histogram) or 0.5 µg of Anti-Mouse CD140a (PDGF Receptor a)Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) andStreptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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参考文献：

1.Nature communicationsIL-17 induced NOTCH1 activation in oligodendrocyte progenitor cells enhances proliferation and inflammatory gene expression."14-1401 was used in Immunofluorescence to demonstrate the crosstalk between the IL-17 and NOTCH1 pathway, regulates Th17-induced inflammatory and proliferative genes promoting demyelinating disease."Authors Wang C,Zhang CJ,Martin BN,Bulek K,Kang Z,Zhao J,Bian G,Carman JA,Gao J,Dongre A,Xue H,Miller SD,Qian Y,Hambardzumyan D,Hamilton T,Ransohoff RM,Li X2.The Journal of clinical investigationPreexisting endothelial cells mediate cardiac neovascularization after injury.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer3.Nature communicationsLaminin regulates PDGFRβ(+) cell stemness and muscle development.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer4.Nature communicationsEndothelial PDGF-CC regulates angiogenesis-dependent thermogenesis in beige fat."14-1401 was used in Flow cytometry/Cell sorting to reveal a paracrine mechanism by which angiogenic endothelial cells modulate adipocyte metabolism."Authors Seki T,Hosaka K,Lim S,Fischer C,Honek J,Yang Y,Andersson P,Nakamura M,Näslund E,Ylä-Herttuala S,Sun M,Iwamoto H,Li X,Liu Y,Samani NJ,Cao Y5.Molecular cancer research : MCRDynamic and nuclear expression of PDGFRα and IGF-1R in alveolar Rhabdomyosarcoma.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer

技术参数

产品应用 Flow；IF；ICC；IF；IHC；IHC (P)；WB；IHC (F)

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