CD11b (activation epitope) Monoclonal Antibody (CBRM1/5), Functional Grade, eBioscience™

货号：16-0113-82,16-0113-38,16-0113-025,16-0113-050

规格：100μg,5mg,25mg,50mg

价格：3434,24089,72266,120443

产品类型：抗体和ELISA

品牌：Thermo Fisher

抗体类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control, Functional Grade, eBioscience™
免疫原性：		用法：	1 µg/test(Flow);Assay-Dependent(FN);Assay-Dependent(Neu)

Description: The CBRM1/5 monoclonal antibody reacts with an activation-specific epitope of human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1 on resting myeloid cells. Through interactions with its ligands, Mac-1 participates in adhesive cell interactions. The epitope recognized by this mAb localizes to the I domain on the alpha chain of Mac-1 very close to the ligand binding site in a region that is widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion Molecule-1 (ICAM-1). It should be noted that low level activation may occur during processing of freshly drawn blood. Therefore the CBRM1.5 antibody may exhibit some binding to Mac-1 in these unstimulated samples. However, higher levels of Mac-1 expression are observed in activated samples when compared to unstimulated cells.Applications Reported: The CBRM1/5 antibody has been reported for use in flow cytometric analysis. CBRM1/5 has also been reported in blocking of CD11b function.Applications Tested: The CBRM1/5 antibody has been tested by flow cytometric analysis of resting and activated normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.Background/Target InformationCD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1.Journal of leukocyte biologyHuman scavenger protein AIM increases foam cell formation and CD36-mediated oxLDL uptake.2014.Authors Amézaga N,Sanjurjo L,Julve J,Aran G,Pérez-Cabezas B,Bastos-Amador P,Armengol C,Vilella R,Escolà-Gil JC,Blanco-Vaca F,Borràs FE,Valledor AF,Sarrias MR2. The Journal of biological chemistryA systemic lupus erythematosus-associated R77H substitution in the CD11b chain of the Mac-1 integrin compromises leukocyte adhesion and phagocytosis.2011.Authors MacPherson M,Lek HS,Prescott A,Fagerholm SC3. Journal of immunology (Baltimore, Md. : 1950)Inflamed lymphatic endothelium suppresses dendritic cell maturation and function via Mac-1/ICAM-1-dependent mechanism.2009.Authors Podgrabinska S,Kamalu O,Mayer L,Shimaoka M,Snoeck H,Randolph GJ,Skobe M4. Cardiovascular research5-Lipoxygenase plays a pivotal role in endothelial adhesion of monocytes via an increased expression of Mac-1.2013.Authors Lee SJ,Choi EK,Seo KW,Bae JU,Kim YH,Park SY,Oh SO,Kim CD5. Cardiovascular research5-Lipoxygenase plays a pivotal role in endothelial adhesion of monocytes via an increased expression of Mac-1.2013.Authors Lee SJ,Choi EK,Seo KW,Bae JU,Kim YH,Park SY,Oh SO,Kim CD

技术参数

产品应用 Flow;Neu;FN

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