CD32 Monoclonal Antibody (6C4 (CD32)), FITC, eBioscience™
货号：11-0329-41,11-0329-42
规格：25 Tests,100 Tests
价格：847,2309
产品类型：流式抗体
品牌：eBioscience
抗原：CD32
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：6C4 (CD32)
荧光染料：FITC
| 类型: | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control, FITC, eBioscience™ |
| 浓度： | 5 µL/Test | 用法： | 5uL（0.25 µg）/test(Flow） |
产品详细信息Description: This 6C4 monoclonal antibody reacts with human CD32 (also known as Fc gammaRII). This antibody recognizes two isoforms of the receptor, Fc gammaRIIA and Fc gammaRIIB. These 40-kDa polymorphic transmembrane glycoproteins are expressed on B cells, granulocytes, monocytes, macrophages, and platelets. Moreover, these receptors are detected on natural killer cells. CD32 enables interaction between Fc gammaRII-expressing cells and opsonized antigen or IgG-containing immune complexes. Bothreceptors exhibit low affinity towards IgG and play a role in inflammation and autoimmune disease. This clone has been reported to inhibit Ig binding in a rosette blocking assay.Applications Reported: This 6C4 (CD32) antibody has been reported for use in flow cytometric analysis.Applications Tested: This 6C4 (CD32) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
| 数据 |
| CD32 Antibody (11-0329-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450(Product # 48-0199-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42)(left) or Anti-Human CD32 FITC (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Journal of neurovirologyImmortalization of primary microglia: a new platform to study HIV regulation in the central nervous system.2.PloS oneMechanisms controlling the effects of bevacizumab (avastin) on the inhibition of early but not late formed corneal neovascularization. |
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