Rat IgG2a kappa Isotype Control, PerCP-Cyanine5.5, eBioscience™

2024-10-17

Rat IgG2a kappa Isotype Control, PerCP-Cyanine5.5, eBioscience™

货号:45-4321-80

规格:25μg

价格:838

产品类型:大鼠同型对照

品牌:Thermo Fisher

物种:其它

宿主:大鼠

抗体亚型:IgG

克隆号:eBR2a

荧光染料:PerCP-Cy5.5

抗体类型:

大鼠同型对照

同型对照:

IgG2a, kappa

免疫原性:

0.2mg/mL

用法:

Assay-Dependent (Ctrl);Assay-Dependent (Flow)

Description: The monoclonal rat IgG2a kappa is useful as an isotype control immunoglobulin.Applications Reported: PerCP-Cy5.5 Rat IgG2a Iso Cntrl has been reported for use in flow cytometric analysis.Applications Tested: Rat IgG2a Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It should be used at the same concentration as the experimental antibody.Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献:

1. Molecular therapy oncolyticsIntratumoral INF-γ triggers an antiviral state in GL261 tumor cells: a major hurdle to overcome for oncolytic vaccinia virus therapy of cancer."45-4321 was used in Flow cytometry/Cell sorting to identify microenvironmental factors, which may be decisive for failure or success of oncolytic VACV therapy."AuthorsKober C,Weibel S,Rohn S,Kirscher L,Szalay AA2. Journal of immunology researchNon-CpG Oligonucleotides Exert Adjuvant Effects by Enhancing Cognate B Cell-T Cell Interactions, Leading to B Cell Activation, Differentiation, and Isotype Switching."45-4321 was used in Flow cytometry/Cell sorting to propose that non-CpG ODN may perform as better adjuvants when a strong antigen-independent immune activation, elicited by CpG ODNs, is undesirable."AuthorsHerbáth M,Papp K,Erdei A,Prechl J3. PloS oneHighly upregulated Lhx2 in the Foxn1-/- nude mouse phenotype reflects a dysregulated and expanded epidermal stem cell niche."45-4321 was used in Flow cytometry/Cell sorting to indicate that the Foxn1(-/-) phenotype has a strong impact on epithelial progeny."AuthorsBohr S,Patel SJ,Vasko R,Shen K,Huang G,Yarmush ML,Berthiaume F4. NatureDirect measurement of local oxygen concentration in the bone marrow of live animals."45-4321 was used in Immunohistochemistry to determine that the absolute pO2 of the bone marrow to be relatively low despite high vascular density."AuthorsSpencer JA,Ferraro F,Roussakis E,Klein A,Wu J,Runnels JM,Zaher W,Mortensen LJ,Alt C,Turcotte R,Yusuf R,Côté D,Vinogradov SA,Scadden DT,Lin CP

技术参数

产品应用 Ctrl;Flow

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