Rat IgG2a kappa Isotype Control, PE-Cyanine7

货号：25-4321-82,25-4321-81

规格：100μg,50μg

价格：1627,1308

产品类型：大鼠同型对照

品牌：eBioscience

物种：其它

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eBR2a

荧光染料：PE-Cy7

抗体类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：		用法：	Assay-Dependent (Ctrl)；Assay-Dependent (Flow)

Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin.Applications Reported: PE-Cy7 Rat IgG2a Iso Cntrl has been reported for use in flow cytometric analysis.Applications Tested: This eBR2a antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at the same concentration as the experimental antibody.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1. Proceedings of the National Academy of Sciences of the United States of AmericaSec61 blockade by mycolactone inhibits antigen cross-presentation independently of endosome-to-cytosol export."25-4321 was used in Flow cytometry/Cell sorting to analyse Sec61's contribution to antigen cross-presentation, ER-associated degradation, and transport of internalised antigens into the cytosol using a newly identified Sec61 blocker."AuthorsGrotzke JE,Kozik P,Morel JD,Impens F,Pietrosemoli N,Cresswell P,Amigorena S,Demangel C2. BioMed research internationalVitamin A Impairs the Reprogramming of Tregs into IL-17-Producing Cells during Intestinal Inflammation."25-4321 was used in Flow cytometry/Cell sorting to study the contribution of different factors to the reprogramming of iTregs into IL-17-producing cells, showing that vitamin A impairs reprogramming during intestinal inflammation."AuthorsTejón G,Manríquez V,De Calisto J,Flores-Santibáñez F,Hidalgo Y,Crisóstomo N,Fernández D,Sauma D,Mora JR,Bono MR,Rosemblatt M3. Comparative immunology, microbiology and infectious diseasesCharacterization of immune modulating functions of γδ T cell subsets in a gnotobiotic pig model of human rotavirus infection."25-4321 was used as a Control in experiments to characterise immune modulating functions of porcine γδ T cell subsets in rotavirus infection."AuthorsWen K,Bui T,Li G,Liu F,Li Y,Kocher J,Yuan L

技术参数

产品应用 Ctrl;Flow

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