Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), PE-Cyanine7, eBioscience™

货号：25-5931-81,25-5931-82

规格：50μg,100μg

价格：1267,2019

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：RB6-8C5

荧光染料：PE-Cy7

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, PE-Cyanine7, eBioscience™
免疫原性：		用法：	0.125 µg/test(Flow）

Description: The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.eBioscience testing indicates that in the bone marrow and lysed whole blood, the antibody clone RB6-8C5 also stains cells that express the highest levels of Ly6c (as defined by staining with antibody clone HK1.4). It is recommended that 1A8-Ly6G (cat. 9668) be used when looking at Ly-6G specific targets.Applications Reported: This RB6-8C5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationGranulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Ly-6G/Ly-6C Antibody (25-5931-82) in Flow Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) eFluor® 450 (Product # 48-0452-82) and 0.125 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 0.125 µg of Anti-Mouse Ly-6G (Gr-1) PE-Cyanine7 (right). Total viable cells were used for analysis.

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参考文献：

1. PLoS geneticsMiR-125a Is a critical modulator for neutrophil development.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. International journal of molecular medicineSilybin attenuates LPS-induced lung injury in mice by inhibiting NF-κB signaling and NLRP3 activation.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. RNA biologyADAR1 deletion induces NFκB and interferon signaling dependent liver inflammation and fibrosis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. Journal of orthopaedic research : official publication of the Orthopaedic Research SocietyDiminished bone regeneration after debridement of posttraumatic osteomyelitis is accompanied by altered cytokine levels, elevated B cell activity, and increased osteoclast activity."25593182 was used in flow cytometry to infer a RANKL-dependent osteoclastogenesis after debridement of osteomyelitis coinciding with elevated B cells and simultaneously decreased osteogenesis"AuthorsWagner JM,Jaurich H,Wallner C,Abraham S,Becerikli M,Dadras M,Harati K,Duhan V,Khairnar V,Lehnhardt M,Behr B5. PloS oneEndothelial Cell-Selective Adhesion Molecule Expression in Hematopoietic Stem/Progenitor Cells Is Essential for Erythropoiesis Recovery after Bone Marrow Injury."25-5931 was used in Flow cytometry/Cell sorting to determine the physiological importance of Endothelial Cell-Selective Adhesion Molecule (ESAM) in erythroid recovery, showing that ESAM is essential for erthryopoiesis recovery following bone marrow injury."AuthorsSudo T,Yokota T,Okuzaki D,Ueda T,Ichii M,Ishibashi T,Isono T,Habuchi Y,Oritani K,Kanakura Y

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