CD326 (EpCAM) Monoclonal Antibody (G8.8), PE-Cyanine7, eBioscience™

货号：25-5791-80

规格：25μg

价格：1267

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：G8.8

荧光染料：PE-Cy7

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, PE-Cyanine7, eBioscience™
免疫原性：		用法：	0.1251 µg/test(Flow）

Description: The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40 (epithelial glycoprotein 40), 17-1A antigen, TACSTD1 (tumor-associated calcium signal transducer 1), and CD326. The immunogen used to generate the G8.8 antibody was the TE-71 thymic epithelial cell line. CD326 is expressed on the majority of epithelial cells, and is considered a pan-carcinoma antigen. CD326 mediates calcium-independent, homophilic, cell-cell adhesion and may function as a growth factor receptor. The antigen is being used as a target for immunotherapy treatment of human carcinomas. CD326 binds LAIR-1 (CD305) and LAIR-2 (CD306) to inhibit cellular activation and inflammation. This epithelial glycoprotein is now recognized as having an important role in tumor biology.Applications Reported: This G8.8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This G8.8 antibody has been tested by flow cytometric analysis of the TE-71 cell line. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationEp-CAM is a ~40 kD glycoprotein, which functions as an epithelial cell adhesion molecule. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions has a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is expressed in the majority of human carcinomas and is a marker for tumors of epithelial lineage.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD326 (EpCAM) Antibody (25-5791-80) in Flow Staining of the TE-71 cell line with 0.06 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (blue histogram) or 0.06 µg of Anti-Mouse CD326 (EpCAM) PE-Cyanine7 (purple histogram). Total viable cells were used for analysis.

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参考文献：

1. International journal of molecular medicineThe C-terminus domain of the hepatitis B virus x protein stimulates the proliferation of mouse foetal hepatic progenitor cells, although it is not required for the formation of spheroids....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Stem cell reportsRegulation of Prostate Development and Benign Prostatic Hyperplasia by Autocrine Cholinergic Signaling via Maintaining the Epithelial Progenitor Cells in Proliferating Status....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Scientific reportsCirculating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. Stem cell reportsLong-Term InVitro Expansion of Salivary Gland Stem Cells Driven by Wnt Signals.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. Stem cell reportsA Serial shRNA Screen for Roadblocks to Reprogramming Identifies the Protein Modifier SUMO2."25-5791 was used in Flow cytometry/Cell sorting to identify potent repressors of somatic cell reprogramming into induced pluripotent stem cells."AuthorsBorkent M,Bennett BD,Lackford B,Bar-Nur O,Brumbaugh J,Wang L,Du Y,Fargo DC,Apostolou E,Cheloufi S,Maherali N,Elledge SJ,Hu G,Hochedlinger K

技术参数

产品应用 Flow

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