MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), APC
货号：17-5958-80,17-5958-82
规格：25μg,100μg
价格：934,2431
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：小鼠
抗体亚型：IgG
克隆号：AF6-88.5.5.3
荧光染料：APC
抗体类型: | 单抗 | 同型对照： | Mouse IgG2a kappa Isotype Control, APC, eBioscience™ |
免疫原性： | | 用法： | 0.125µg/test(Flow） |
Description: This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed.Applications Reported: This AF6-88.5.5.3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This AF6-88.5.5.3 antibody has been tested by flow cytometric analysis on C57Bl/6 splenocytes. This antibody can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThis protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| MHC Class I (H-2Kb) Antibody (17-5958-82) in Flow Staining of C57BL/6 (left) and BALB/c (right) splenocytes with 0.06 µg of Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (blue histogram) or 0.06 µg of Anti-Mouse MHC Class I (H-2Kb) APC (purple histogram). Total viable cells were used for analysis. |
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参考文献： |
| 1. Nature communicationsDeletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Clinical cancer research : an official journal of the American Association for Cancer ResearchHDAC Inhibitors Enhance T-Cell Chemokine Expression and Augment Response to PD-1 Immunotherapy in Lung Adenocarcinoma."17-5958 was used in Flow cytometry/Cell sorting to test whether strategies that increase T-cell infiltration to tumours can be efficacious in enhancing immunotherapy response."AuthorsZheng H,Zhao W,Yan C,Watson CC,Massengill M,Xie M,Massengill C,Noyes DR,Martinez GV,Afzal R,Chen Z,Ren X,Antonia SJ,Haura EB,Ruffell B,Beg AA3. OncotargetAnti-angiogenic efficacy of 5'-triphosphate siRNA combining VEGF silencing and RIG-I activation in NSCLCs.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. VaccinesEpitope Prediction Assays Combined with Validation Assays Strongly Narrows down Putative Cytotoxic T Lymphocyte Epitopes."17-5958 was used in Flow cytometry/Cell sorting to investigate the most promising C57BL/6 mouse-restricted Hepatitis C virus (HCV) MHC class I epitopes and validate these epitopes in vitro and in vivo."AuthorsIp PP,Nijman HW,Daemen T5. Journal of immunology (Baltimore, Md. : 1950)Oxidized lipids block antigen cross-presentation by dendritic cells in cancer.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer |
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