CD117 (c-Kit) Monoclonal Antibody (ACK2), APC

货号：17-1172-82

规格：100μg

价格：2722

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：ACK2

荧光染料：APC

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	0.06 µg/test(Flow）

Description: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.Applications Reported: The ACK2 antibody has been reported for use in flow cytometric analysis.Applications Tested: The ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target Informationc-Kit, also known as CD117 and stem cell factor receptor, is a 145 kDa transmembrane tyrosine kinase encoded by the c-Kit proto-oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. c-Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Phosphorylation at tyrosine 703 of c-Kit allows binding of Grb2 and activation of the Ras-Raf-ERK1 and 2 signaling pathway.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD117 (c-Kit) Antibody (17-1172-82) in Flow Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.03 µg of Rat IgG2b K Isotype Control APC (Product # 17-4031-82) (left) or 0.03 µg of Anti-Mouse CD117 (c-Kit) APC (right). Total viable cells were used for analysis.

推荐产品：

流式抗体细胞周期检测细胞凋亡检测细胞增殖活性检测

参考文献：

1. PloS oneRegulation of Reactive Oxygen Species and the Antioxidant Protein DJ-1 in Mastocytosis."17-1172 was used in Flow cytometry/Cell sorting to propose consideration of IL-6 blockade as a potential adjunctive therapy in the treatment of patients with advanced mastocytosis."AuthorsKim DK,Beaven MA,Kulinski JM,Desai A,Bandara G,Bai Y,Prussin C,Schwartz LB,Komarow H,Metcalfe DD,Olivera A2. PloS oneCharacterisation of Neutropenia-Associated Neutrophil Elastase Mutations in a Murine Differentiation Model In Vitro and In Vivo."17-1172 was used in Flow cytometry/Cell sorting to investigate the molecular mechanisms for neutrophil survival and maturation defects caused by mutant neutrophil elastase protein."AuthorsWiesmeier M,Gautam S,Kirschnek S,Häcker G3. Nature communicationsRegulation of B-cell development and tolerance by different members of the miR-17∼92 family microRNAs.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. CellCore Circadian Clock Genes Regulate Leukemia Stem Cells in AML.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. Cancer scienceNovel oral transforming growth factor-β signaling inhibitor EW-7197 eradicates CML-initiating cells."17-1172 was used in Flow cytometry/Cell sorting to indicate that EW-7197 may be a promising candidate for a new combination therapeutic that can greatly benefit CML patients."AuthorsNaka K,Ishihara K,Jomen Y,Jin CH,Kim DH,Gu YK,Jeong ES,Li S,Krause DS,Kim DW,Bae E,Takihara Y,Hirao A,Oshima H,Oshima M,Ooshima A,Sheen YY,Kim SJ,Kim DK

技术参数

产品应用 Flow

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://www.biorod.cn/thread-17094.htm