CD16/CD32 Monoclonal Antibody (93), APC

货号：17-0161-81,17-0161-82

规格：50μg,100μg

价格：1721,3146

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：93

荧光染料：APC

抗体类型:	单抗	同型对照：	IgG2a, lambda
免疫原性：		用法：	0.25 µg/test(Flow）

Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.Applications Reported: The 93 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD16/CD32 Antibody (17-0161-81) in Flow Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.125 µg of Anti-Mouse CD16/CD32 APC (filled histogram). Total viable cells were used for analysis.

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参考文献：

1. Nature communicationsRational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. NatureMyeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Journal for immunotherapy of cancerAgonist anti-GITR monoclonal antibody and stereotactic radiation induce immune-mediated survival advantage in murine intracranial glioma....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. Molecular medicine reportsGenomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model."17-0161 was used in Flow cytometry/Cell sorting to understand the virological features of enterovirus 71 and the host responses resulting from enterovirus 71 infection."AuthorsYue Y,Li P,Song N,Li B,Li Z,Guo Y,Zhang W,Wei MQ,Gai Z,Meng H,Wang J,Qin L5. Scientific reportsFunctions of miR-146a and miR-222 in Tumor-associated Macrophages in Breast Cancer."17-0161 was used in Flow cytometry/Cell sorting to study the role of endogenous miRNAs in controlling the polarisation and function of tumour-associated macrophages in breast cancer."AuthorsLi Y,Zhao L,Shi B,Ma S,Xu Z,Ge Y,Liu Y,Zheng D,Shi J

技术参数

产品应用 Flow

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