TCR gamma/delta Monoclonal Antibody (eBioGL3 (GL-3, GL3)), PE-Cyanine5

货号：15-5711-81,15-5711-82

规格：50 μg,100μg

价格：1577,2630

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：仓鼠

抗体亚型：IgG

克隆号：eBioGL3 (GL-3, GL3)

荧光染料：PE-Cy5

抗体类型:	单抗	同型对照：	Armenian Hamster IgG Isotype Control, PE-Cyanine5, eBioscience™
免疫原性：		用法：	0.5 µg/test(Flow）

Description: The GL3 monoclonal antibody reacts with the gamma delta T-cell Receptor complex (TCR) on all gamma delta TCR bearing T cells, but it does not react with alpha beta TCR. The gamma delta TCR is present on cells in the thymus, epidermis, epithelial lining of the intestine, peritoneal cavity, and lymphoid tissues.Applications Reported: This GL3 (GL-3) antibody has been reported for use in flow cytometric analysis.It is recommended to prestain cells with anti-mouse CD16/32 (cat. 14-0161) to prevent non-specific Fc-mediated binding of the GL3 monoclonal antibody.Applications Tested: This eBioGL3 (GL-3, GL3) antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationTCR gamma/delta (T-cell receptor gamma/delta) are specialized T-cells in the immune system. The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory auto-destructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). However, a small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

TCR gamma/delta Antibody (15-5711-82) in Flow Staining of C57Bl/6 lymph node cells with Anti-Mouse CD3e PE (Product # 12-0031-82) and 0.5 µg of Armenian Hamster IgG Isotype Control PE-Cyanine5 (Product # 15-4888-82) (left) or 0.5 µg of Anti-Mouse gamma delta TCR PE-Cyanine5 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. Nature communicationsIL-1 receptor antagonist-deficient mice develop autoimmune arthritis due to intrinsic activation of IL-17-producing CCR2(+)Vγ6(+)γδ T cells....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Nature immunologyNatural and inducible TH17 cells are regulated differently by Akt and mTOR pathways."15-5711 was used in Flow cytometry/Cell sorting to define mechanisms regulating nTH17 cell development and reveal the roles of Akt and mTOR in shaping subsets of T cells."AuthorsKim JS,Sklarz T,Banks LB,Gohil M,Waickman AT,Skuli N,Krock BL,Luo CT,Hu W,Pollizzi KN,Li MO,Rathmell JC,Birnbaum MJ,Powell JD,Jordan MS,Koretzky GA

技术参数

产品应用 Flow

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