CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), PE-Cyanine5
货号：15-0083-81
规格：50μg
价格：1363
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2b, kappa
克隆号：eBioH35-17.2 (H35-17.2)
荧光染料：PE-Cy5
抗体类型: | 单抗 | 同型对照： | Rat IgG2b kappa Isotype Control, PE-Cyanine5, eBioscience™ |
免疫原性： | | 用法： | 0.125 µg/test(Flow） |
Description: The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells.Applications Reported: This eBioH35-17.2 (H35-17.2) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioH35-17.2 (H35-17.2) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| CD8b Antibody (15-0083-81) in Flow Staining of C57Bl/6 splenocytes with Anti-Mouse CD8a FITC (Product # 11-0081-82) and 0.06 µg of Anti-Mouse CD8b PE-Cyanine5. Cells in the lymphocyte gate were used for analysis. |
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参考文献： |
| 1. Nature communicationsIntratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Journal of leukocyte biologyA novel Cd8-cis-regulatory element preferentially directs expression in CD44hiCD62L+ CD8+ T cells and in CD8αα+ dendritic cells."15-0083 was used in Flow cytometry/Cell sorting to identify a novel Cd8 enhancer that directs expression in CD44(hi)CD62L(+) CD8(+) T cells, including innate-like and antigen-specific effector/memory CD8(+) T cells and in CD8αα(+) DCs."AuthorsSakaguchi S,Hombauer M,Hassan H,Tanaka H,Yasmin N,Naoe Y,Bilic I,Moser MA,Hainberger D,Mayer H,Seiser C,Bergthaler A,Taniuchi I,Ellmeier W3. PloS oneDeletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. Nature communicationsMetastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. PloS oneGranulocytes and vascularization regulate uterine bleeding and tissue remodeling in a mouse menstruation model."15-0083 was used in Flow cytometry/Cell sorting to investigate mechanisms underlying pathogenesis of menstrual disorders, showing that granulocytes and vascularisation regulate uterine bleeding and tissue remodelling."AuthorsMenning A,Walter A,Rudolph M,Gashaw I,Fritzemeier KH,Roese |
QQ:1749072012
