Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 790/山羊抗小鼠IgG (H+L)高交叉吸附二抗，Alexa Fluor 790

货号：A11357

规格：1 mg

价格：3553

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 790

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抗体类型:	荧光二抗	同型对照：
浓度：	2 mg/mL	用法：	1-10 µg/mL（Flow）；1-10 µg/mL(ICC)；1-10 µg/mL(IF)；1:5,000:1-20,000(WB)

产品详细信息This secondary antibody is designed for fluorescent Western blot detection on various near-infrared fluorescence instruments. This antibody can be used for multi-color and multiplexing detection when using other antibodies conjugated to compatible Alexa Fluor™ dyes and wavelengths. Other applications of this antibody include immunofluorescent and fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities. 靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A11357) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-87 MG (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 0.5 µg/mL) and detected using Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 790 (Product # A11357) at dilutions 1:5,000 (Fig. 1), 1:10,000 (Fig. 2) and 1:20,000 (Fig. 3). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Fluorescent detection was performed using the Odyssey® Fc imaging system (Li-cor Biosciences).

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参考文献：

1.Nature communications Correspondence: Oncogenic MYC persistently upregulates the molecular clock component REV-ERBα. 2.Proceedings of the National Academy of Sciences of the United States of America Loss of O-GlcNAc glycosylation in forebrain excitatory neurons induces neurodegeneration. 3.The Journal of experimental medicine Notch activation drives adipocyte dedifferentiation and tumorigenic transformation in mice. 4.eLife Stage-specific effects of Notch activation during skeletal myogenesis.

技术参数

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