Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor 647
货号：A27018
规格：1mg
价格：3306
产品类型：荧光二抗
品牌：Thermo Fisher
物种：山羊
宿主：兔
抗体亚型：IgG
荧光染料：Alexa Fluor 647
抗体类型: | 荧光二抗 | 同型对照： | IgG |
免疫原性： | Recombinant full-length protein | 用法： | 1-10 μg/mL (ICC)；1-10 μg/mL (IF) |
The sensitivity and specificity of each lot is confirmed using ELISA.Minimal cross-reactivity with mouse, rat, human, bovine, guinea pig, and donkey IgG is observed.Background/Target InformationSuperclonal™ secondary antibodies are developed to be highly specific for both heavy and light chains of IgG. Utilizing the power of recombinant technology and multiple phenotypic screens, Superclonal™ antibodies are selected for enhanced specificity to the target species IgG. These recombinant secondary antibodies show similar performance as traditional polyclonal secondary antibodies in common immunoassay applications, with minimal cross-reactivity to other commonly-used species. In addition, their recombinant animal-free-origin helps ensures lot-to-lot consistency, reducing the need to optimize each lot in relevant assays.Superclonal™ secondary antibodies are designed to allow scientists to achieve the accurate results they need, without some of the concerns that can impact traditional polyclonal secondary antibodies.
数据 |
| Goat IgG (H+L) Secondary Antibody (A27018) in IFSmooth muscle Actin (green) in U20S human osteocarcinoma cells was labeled with Goat anti-alpha-smooth muscle Actin primary antibody and visualized using Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 647 conjugate (Product # A27018) (0.4 µg/mL, 1:2500). Nuclei (blue) were stained with SlowFade® Gold Antifade Mountant using DAPI (Product # S36938) (1:50). To demonstrate multiplexing Apoptosis Inducing Factor (red) was labeled with anti-AIF mouse primary antibody (Product # 45-6200) and visualized with Rabbit anti-Mouse IgG (H+L), Alexa Fluor® 488 conjugate (Product # A-11059) (1:400). |
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参考文献： |
| 1.Nature neuroscienceAge-related myelin degradation burdens the clearance function of microglia during aging."A27018 was used in immunohistochemistry to characterize age-related myelin fragmentation"AuthorsSafaiyan S,Kannaiyan N,Snaidero N,Brioschi S,Biber K,Yona S,Edinger AL,Jung S,Rossner MJ,Simons M |
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