SYTOX™ Green Dead Cell Stain死细胞染料488nm激发 用于流式

2024-10-17

SYTOX™ Green Dead Cell Stain死细胞染料488nm激发 用于流式

货号:S34860

规格:1 mL

价格:4631

产品类型:细胞增殖活性检测

品牌:Thermo Fisher

物种:其它

宿主:其它

抗体亚型:其它

荧光染料:其它

SYTOX™ Green Dead Cell Stain is a bright, easy-to-use nucleic acid stain for distinguishing dead from live cells in flow cytometry assays. It is excitable at 488 nm with an emission profile similar to FITC, and has been tested in an assortment of cell types.参考不可固定的死活细胞染料,选择适合您流式实验的试剂。You get these advantages with SYTOX™ Green Dead Cell Stain:• Bright Signal—Easily distinguishes dead cells from live cells.• Rapid Staining—Single step, No Wash Assay to assess viability of unfixed cells.• Validation—in Jurkat, ReH, CHO, Kg1a, 3T3, HeLa, HEK (primary cells), and lysed whole blood.• Optimization—Dye concentration has been optimized for use in flow cytometry.• Versatility—Tested in a variety of experimental applications.A bright stain in an easy to use, no wash assaySYTOX™ Green Dead Cell Stain is a cell-impermeant nucleic acid stain. By flow cytometry, dead cells can be easily distinguished from live cells by the increased fluorescent signal due to dsDNA binding of SYTOX™ Green (Fig 1). Because it is non-fluorescent in aqueous media, SYTOX™ Green Dead Cell Stain can be applied to cells and visualized with minimal background staining and without an additional wash step.Validated in flow cytometrySYTOX™ Green is designed for flow cytometry using the 488 nm blue laser with emission in the 530/30 BP filter (similar to FITC and GFP). The dye concentration has been optimized for samples with concentrations ranging from 1x104 to 1x106 cells per ml.SYTOX™ Green Cell Stain has been validated in several cell types including:• Jurkat• ReH• CHO• Kg1a• 3T3• HeLa• HEK (primary cells)• Lysed whole bloodSYTOX™ Green Dead Cell Stain has been tested in several applications including:• 细胞凋亡• 细胞周期• 细胞增殖•免疫表型分析Part of the SYTOX™ family of cell viability stainsSYTOX™ Dead Cell Stains come in an assortment of excitation/emission characteristics and have been incorporated into a number of assays for apoptosis, cell viability, and metabolism.• SYTOX™ Blue Dead Cell Stain (S34857): For the 405 nm violet laser.• SYTOX™ AADvanced™ Dead Cell Stain (S10274, S10309): For the 488 nm laser with red emission.• SYTOX™ Red Dead Cell Stain (S34859): For the 633/5 nm red laser.• SYTOX™ Orange Dead Cell Stain (S34861): For the 532 nm green laser.• SYTOX™ Dead Cell Stain Sampler Kit (S34862).Related Link• Learn about all SYTOX™ Products.
相关产品:
细胞周期检测细胞凋亡检测细胞增殖活性检测
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