eGFP Monoclonal Antibody (F56-6A1.2.3)/eGFP标签抗体

货号： MA1-952

规格：100 µg

价格：2753

产品类型：标签抗体

品牌：Thermo Fisher

物种：Tag

宿主：小鼠

抗体亚型：其它

荧光染料：其它

点击Invitrogen标签抗体集，查看更多标签抗体

抗体类型:	单抗	同型对照：	IgG2b
免疫原性：	Full length enhanced green fluorescent protein (eGFP)	用法：	1:20-1:100（ICC）；1:20-1:100（IF）；1:1000(WB)

Product Specific InformationMA1-952 contains 100 µg of in vitro produced, Protein A purified IgG (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide.MA1-952 detects eGFP and eGFP tagged proteins.MA1-952 has been successfully used in Western blot and immunofluorescence applications.The MA1-952 immunogen is full length enhanced green fluorescent protein (eGFP).Background/Target InformationThe jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organinsms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcelluclar compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used etensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

eGFP Antibody (MA1-952) in IFImmunofluorescent analysis of eGFP using either natural fluorescence (green) or an anti-eGFP antibody (red) in U2OS cells transfected with an EGFR-eGFP fusion protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature and blocked with 0.3% BSA for 15 minutes at room temperature. Cells were probed with a GFP Epitope Tag monoclonal antibody (Product # MA1-952) at a dilution of 1:20 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 550 goat anti-mouse IgG secondary antibody (Product # 84540) at a dilution of 1:200 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan Instrument at 20X magnification. eGFP Antibody (MA1-952) in WBWestern blot analysis of GFP was performed by loading various amounts of recombinant GFP per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a GFP monoclonal antibody (Product # MA1-952) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 32430) at a dilution of 1:15,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34087).

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参考文献：

1.Journal of cellular biochemistryc-Myc-Dependent Cell Competition in Human Cancer Cells."MA1952 was used in western blot to design a c-Myc (human homolog)-dependent in vitro cell competition model of human cancer cells in Drosophila"AuthorsPatel MS,Shah HS,Shrivastava N2.Journal of cellular and molecular medicineCx31.1 acts as a tumour suppressor in non-small cell lung cancer (NSCLC) cell lines through inhibition of cell proliferation and metastasis."MA1-952 was used in western blot to examine the role of Cx31.1 in non-small cell lung cancer"AuthorsZhang D,Chen C,Li Y,Fu X,Xie Y,Li Y,Huang Y

技术参数

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