GAPDH Loading Control Monoclonal Antibody (GA1R)/GAPDH抗体

货号：MA5-15738,MA5-15738-1MG

规格：100 ug,1 mg

价格：3542,13782

产品类型：内参抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

荧光染料：其它

点击Invitrogen内参抗体集，查看更多内参抗体

类型:	单抗	同型对照：	IgG1
免疫原性：	Recombinant GAPDH	用法：	2 µg/test(flow);1:20-1:200(ICC);1:20-1:200(IF);1:50-1:500(IHC(P));1:1000-10000(WB)

Product Specific InformationThis antibody detects GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, hamster, and chicken samples.MA5-15738 has been successfully used in Western blot, ICC, IF, IHC (P), FACS and ELISA.MA5-15738 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.Background/Target InformationGAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

GAPDH Loading Control Antibody (MA5-15738) in FlowFlow cytometry analysis of GAPDH in MCF-7 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a GAPDH loading control antibody (Product # MA5-15738) at a dilution of 2 µg/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis. GAPDH Loading Control Antibody (MA5-15738) in IFImmunofluorescent analysis of GAPDH (green) showing staining in the in the cytoplasm of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a GAPDH loading control antibody (Product # MA5-15738) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x. GAPDH Loading Control Antibody (MA5-15738) in IHC (P)Immunohistochemistry analysis of GAPDH showing staining in the nucleus and cytoplasm of paraffin-embedded human breast carcinoma (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a GAPDH monoclonal antibody (Product # MA5-15738) diluted in 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.Cell death discoveryFoxO1 interacts with transcription factor EB and differentially regulates mitochondrial uncoupling proteins via autophagy in adipocytes."MA5-15738 was used in western blot to elucidate how FoxO1 regulates mitochondrial uncoupling proteins"AuthorsLiu L,Tao Z,Zheng LD,Brooke JP,Smith CM,Liu D,Long YC,Cheng Z2.The American journal of sports medicineThe Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration."MA515738 was used in western blot to distinguish the effects of the non-neutrophil-containing plasma fractions on human skeletal muscle myoblast differentiation"AuthorsMiroshnychenko O,Chang WT,Dragoo JL3.AutophagyA novel autophagy modulator 6-Bio ameliorates SNCA/α-synuclein toxicity."MA515738 was used in western blot to describe the effects of 6-Bio using a preclinical model of Parkinson disease"AuthorsSuresh SN,Chavalmane AK,Dj V,Yarreiphang H,Rai S,Paul A,Clement JP,Alladi PA,Manjithaya R4.Virology journalSmall-size recombinant adenoviral hexon protein fragments for the production of virus-type specific antibodies."MA5-15738 was used in Western Blotting to develop a novel method for the production of hyper-variable regions 1-6 of the adenoviral hexon protein and specific antibody generation."AuthorsPacesa M,Hendrickx R,Bieri M,Flatt JW,Greber UF,Hemmi S5.Nature communicationsInfluenza virus genome reaches the plasma membrane via a modified endoplasmic reticulum and Rab11-dependent vesicles."MA5-15738 was used in Western Blotting to reveal that the endomembrane organelle that is primarily involved in the transport of vRNPs is the ER."Authorsde Castro Martin IF,Fournier G,Sachse M,Pizarro-Cerda J,Risco C,Naffakh N

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://www.biorod.cn/thread-17621.htm