ArC™ Amine Reactive Compensation Bead Kit (for use with LIVE/DEAD™ Fixable dead cell stain kits)

货号：A10628,A10346

规格：1 kit,1 kit

价格：1707,5281

产品类型：检测试剂盒

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

ArC™ Amine Reactive Compensation Bead Kit is a bead-based compensation tool specifically optimized for use with LIVE/DEAD™ Fixable dead cell stain kits.• Ready-to-use control• Eliminates the hassle of heat-treating cells as a control, saving precious sample• Enables accurate and consistent resultsView a selection guide for all fixable viability dyes for flow cytometry.Ready-to-use controlUnlike other methods that require sample preparation to make compensation controls using cellular material, the ArC™ Amine Reactive Compensation Bead Kit is ready to use out of the dropper bottle. The kit includes two types of specially modified polystyrene microspheres that have been optimized to react with the amine-reactive dye in the LIVE/DEAD™ Fixable dead cell stain kits. The ArC™ reactive beads (Component A) bind any of the amine-reactive dyes and provide a positive signal; the ArC™ negative beads (Component B) have no reactivity and provide a negative compensation control. This ready-to-use product preserves the original sample material for experiments; precious sample is not used for the creation of compensation controls.Accurate and consistent resultsUnlike biological controls, which can have variable expression levels depending upon disease or development state, the Arc™ beads provide a bright, consistent signal (see figure below). Consistency in the staining pattern of compensation controls is important for obtaining the right compensation value. If the signal varies with the control material used, the compensation value can be affected, leading to erroneous results.How it worksOne drop of beads is added to a test tube containing LIVE/DEAD™ Fixable dead cell stain. After incubation with amine-reactive dye, the beads are washed in staining buffer, a drop of negative control beads is added (if required), and the beads are resuspended and analyzed by flow cytometry. The two components provide a distinct positive bead that is at least 50-fold brighter than the negative beads, which can be used to set compensation.Other compensation productsBesides the ArC™ Amine Reactive Compensation Beads, Life Technologies also offers the AbC™ Anti-Mouse Bead Kit for capture of mouse anti-human antibodies and AbC™ Anti-Rat/Hamster Bead Kit for capture of rat anti-mouse or hamster anti-mouse antibodies.

数据：

Beads from the ArC™ Bead Kit (A 10346) were stained with LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit (V34955) and analyzed by flow cytometry.A mixture of the positive and negative beads in the ArC™ Bead Kit (A 10346) were stained with LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit (V34955), washed, and analyzed using a flow cytometry equipped with a 405/7nm laser with 450/50 nm band pass filter. Histogram shows postive and negative peaks clearly distinguishable.

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▪细胞周期检测▪细胞凋亡检测▪细胞增殖活性检测

技术参数

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