| Characterization and differentiation of iPSC colonies generated on xeno-free conditions. Colonies were stained for pluripotent markers: NANOG (b), SSEA4 (c), SOX2 (e), Tra1-60 (f), and phase ((a) and (d)). (B) These colonies were differentiated in vitro into embryoid bodies (EBs) comprising of the three embryonic germ layers: endoderm marker alpha fetoprotein (AFP) (g), mesodermal marker smooth muscle actin (SMA) (h), and ectoderm marker Beta 3-tubulin (Beta III) (i).TRA-1-60 Antibody (41-1000) in IFIndirect immunofluorescent analysis of SHEFF4 human ES cells using Zymed Ms anti-TRA-1-60 (Product # 41-1000). Image courtesy of Peter Andrews, Ph.D., University of Sheffield, Sheffield, UK.TRA-1-60 Antibody (41-1000) in WBWestern blot analysis of human embryonal carcinoma EC1003.7 cell lysates using Zymed (R) Ms anti-TRA-1-60 (Product # 41-1000). |
| 1.Stem cells and developmentApplications of quantitative polymerase chain reaction protein assays during reprogramming.AuthorsRuff D,MacArthur C,Tran H,Bergseid J,Tian J,Shannon M,Chen SM,Fontes A,Laurent L,Swartzman E,Taliana A,Rao M,Lieu PT2.Frontiers in cellular neuroscienceGene expression profiling for human iPS-derived motor neurons from sporadic ALS patients reveals a strong association between mitochondrial functions and neurodegeneration.3.Nature protocolsEfficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells."41-1000 was used in western blot to describe a method to generate cystic fibrosis transmembrane conductance regulator protein-expressing airway epithelial cells from human pluripotent stem cells"AuthorsWong AP,Chin S,Xia S,Garner J,Bear CE,Rossant J4.Nature methodsIsolation of human iPS cells using EOS lentiviral vectors to select for pluripotency."41-1000 was used in flow cytometry and immunocytochemistry to report the first selection system for the isolation of human induced pluripotent stem cells"AuthorsHotta A,Cheung AY,Farra N,Vijayaragavan K,Séguin CA,Draper JS,Pasceri P,Maksakova IA,Mager DL,Rossant J,Bhatia M,Ellis J5.International journal of cancerComparative analysis of cell surface antigens expressed by cell lines derived from human germ cell tumours.AuthorsAndrews PW,Casper J,Damjanov I,Duggan-Keen M,Giwercman A,Hata J,von Keitz A,Looijenga LH,Millán JL,Oosterhuis JW,Pera M,Sawada M,Schmoll HJ,Skakkebaek NE,van Putten W,Stern P |
QQ:1749072012