LIN28A Monoclonal Antibody (14E6-4E6), HRP

货号：MA1-016-HRP

规格：100 µL

价格：4809

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：LIN28A

物种：人/小鼠

宿主：小鼠

抗体亚型：其它

克隆号：14E6-4E6

荧光染料：HRP

类型:	单抗	同型对照：	IgG2a
免疫原性：	Full-length human recombinant protein expressed in bacteria	用法：	1:250-1:1000(WB)

Product Specific InformationMA1-016-HRP has been successfully used in Western blot applications on human and mouse samples.Target InformationLIN28A/B are expressed in various cancers and ES cells. LIN28 is a cytoplasmic protein, that acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. LIN28 is a negative regulator of microRNA (miRNA) biogenesis by specifically binding the precursor of let-7 (pre-let-7). Let-7 miRNAs have been implicated in repression of oncogenes such as ras and myc. LIN28 expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

LIN28A Antibody (MA1-016-HRP) in WBWestern blot analysis of Lin28 was performed by loading 50 µg of NCCIT and HeLa cell lysates per well onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated Lin28 monoclonal antibody (Product # MA1-016-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

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