SSEA3 Monoclonal Antibody (eBioMC-631 (MC-631)), eBioscience™

货号：14-8833-80

规格：25 µg

价格：1575

产品类型：流式抗体

品牌：eBioscience

抗原：SSEA3

物种：人/小鼠

宿主：大鼠

抗体亚型：IgM

克隆号：eBioMC-631 (MC-631

荧光染料：其它

类型:	单抗	同型对照：	IgM
免疫原性：		用法：	0.5 µg/test(Flow)

Product Specific InformationDescription: The eBioMC-631 (MC-631) monoclonal antibody reacts with the carbohydrate moiety galactosylgloboside on Stage-specific embryonic antigen-3 (SSEA-3). Expression is restricted to the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES) and down regulated following differentiation.Applications Reported: This eBioMC-631 (MC-631) antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry.Applications Tested: This eBioMC-631 (MC-631) antibody has been tested by flow cytometric analysis of the 2102Ep cell line. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Target InformationSSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

SSEA3 Antibody (14-8833-80) in FlowStaining of the 2102Ep cell line with 0.5 µg of Rat IgM, K Isotype Control Purified (Product # 14-4341-82) (open histogram) or 0.5 µg of Anti-Human/Mouse SSEA-3 Purified (filled histogram) followed by Anti-Rat IgM PE (Product # 12-0990-82).Total viable cells were used for analysis.

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参考文献：

1.Methods in molecular biology (Clifton, N.J.) In Vitro Modeling of Alcohol-Induced Liver Injury Using Human-Induced Pluripotent Stem Cells. "14-8833 was used in Flow cytometry/Cell sorting to study the effects of alcohol exposure on multistage hepatic cells to model the early stages of liver tissue injury associated with alcoholic liver disease." AuthorsTian L,Prasad N,Jang YY 2.The Journal of biological chemistry Sialyl-lactotetra, a novel cell surface marker of undifferentiated human pluripotent stem cells. "14-8833 was used in Flow cytometry/Cell sorting to characterise acid glycosphingolipids isolated from human embryonic stem cells, revealing a novel marker of undifferentiated cells, sialyl-lactotetra." AuthorsBarone A,Säljö K,Benktander J,Blomqvist M,Månsson JE,Johansson BR,Mölne J,Aspegren A,Björquist P,Breimer ME,Teneberg S

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