TRA-1-60 (Podocalyxin) Monoclonal Antibody (TRA-1-60), PE, eBioscience™

货号：12-8863-82,12-8863-80

规格：100 µg,25 µg

价格：2055,0

产品类型：流式抗体

品牌：eBioscience

抗原：TRA-1-60

物种：人

宿主：小鼠

抗体亚型：IgM

克隆号：TRA-1-60

荧光染料：PE

类型:	单抗	同型对照：	IgM
免疫原性：		用法：	1 µg/test(Flow)

Product Specific InformationDescription: The TRA-1-60 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation. Contrary to early reports that the TRA-1-60 epitope can be destroyed by neuraminidase digestion, new strudies have shown that TRA-1-60 recognizes a neurominidase resistent antigen. The TRA-1-60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.Applications Reported: This TRA-1-60 antibody has been reported for use in flow cytometric analysis.Applications Tested: This TRA-1-60 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationTRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

TRA-1-60 (Podocalyxin) Antibody (12-8863-82) in FlowStaining of 2102Ep human embryonal carcinoma cell line with 0.5 µg of Mouse IgM Isotype Control PE (Product # 12-4752) (open histogram) or 0.5 µg of Anti-Human TRA-1-60 PE (filled histogram). Total viable cells were used for analysis.

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参考文献：

1.Cell stem cellAnalysis of Transcriptional Variability in a Large Human iPSC Library Reveals Genetic and Non-genetic Determinants of Heterogeneity.AuthorsCarcamo-Orive I,Hoffman GE,Cundiff P,Beckmann ND,D'Souza SL,Knowles JW,Patel A,Papatsenko D,Abbasi F,Reaven GM,Whalen S,Lee P,Shahbazi M,Henrion MYR,Zhu K,Wang S,Roussos P,Schadt EE,Pandey G,Chang R,Quertermous T,Lemischka I

技术参数

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