EconoTaq PLUS 2X Master Mix，2×PCR预混合液
货号：30035-1,30035-2
规格：500 rxns,1000 rxns
价格：2172,3604
产品类型：PCR 及DNA聚合酶
品牌：Lucigen
| EconoTaq PLUS 2X Master Mix is a ready-to-use PCR master mix containing agarose gel loading
buffer. A PCR reaction is set up simply by combining the EconoTaq PLUS Master Mix with template
DNA, primers, and water. After the reaction is complete, it can be loaded directly onto an agarose gel.
All other necessary components are provided in the Master Mix, which contains the following: 0.1
units/µL of EconoTaq DNA Polymerase, Reaction Buffer (pH 9.0), 400 µM dATP, 400 µM dGTP, 400 µM
dCTP, 400 µM dATP, 3 mM MgCl2, and a proprietary PCR Enhancer/Stabilizer. For difficult templates,
such as those containing GC content up to 75%, it has been found that EconoTaq PLUS Master Mix
performs well with denaturation temperatures up to 98 °C.EconoTaq PLUS 2X Master Mix是一种即用型PCR预混液，含有dNTP和PCR Enhancer。它具有出色的性能和价值，是常规PCR的理想选择。 |
| 优点： |
| ▪Outstanding performance and value are perfect for routine PCR；▪Can be cycled up to 98°C to amplify the most challenging GC-rich templates others cannot；▪Ready-to-use PCR reaction master mixes, contain dNTPs and PCR Enhancer. |
| 数据： |
| Figure 1.EconoTaq PLUS GREEN Master Mix vs. GoTaq® Green master mix (Promega) in PCR. PCR was performed according to the manufacturer’s recommendations using primers specific for the indicated genes.Panel A,0.7 kb prokaryotic single-stranded DNA binding protein (genomic DNA);Panel B,2.7 kb prokaryotic DNA polymerase A (genomic DNA);Panel C,4.5 kb gene (plasmid DNA).Figure 2.GC-rich regions from human genomic DNA were PCR amplified using 98° C denaturation.Figure 3.Easy visualization with EconoTaq PLUS GREEN. During electrophoresis, the green loading color separates into a slow-moving blue dye and a fast-moving yellow dye. |
技术参数 产品优点 - Outstanding performance and value are perfect for routine PCR. - Can be cycled up to 98°C to amplify the most challenging GC-rich templates others cannot. - Ready-to-use PCR reaction master mixes, contain dNTPs and PCR Enhancer. - Non-proofreading polymerase - EconoTaq PLUS GREEN contains tracking dyes for gel electrophoresis
产品应用 - Use EconoTaq PLUS 2X Master Mix when the PCR products will be analyzed by absorbance or fluorescence excitation, to avoid possible interference by the dyes. - DNA模板的PCR和多重PCR扩增
推荐用于 Routine PCR
组成成分 EconoTaq PLUS 2X Master Mix contains all of the components necessary to performPCR amplification, except for template and primers. The Master Mix also contains agarose gel loading buffer.
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