Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PE, eBioscience™

货号：12-5981-82,12-5981-81,12-5981-83

规格：100 µg,50 µg,200 µg

价格：2119,1148,2892

产品类型：流式抗体

品牌：eBioscience

抗原：Sca-1

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：D7

荧光染料：PE

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™
浓度：	0.2 mg/mL	用法：	0.25 µg/test(Flow)

Product Specific InformationDescription: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationLy-6A/E molecules are implicated in the regulation of T cell activation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Ly-6A/E (Sca-1) Antibody (12-5981-82) in FlowStaining of C57Bl/6 bone marrow cells with Mouse Hematopoietic Lineage Cocktail eFluor® 450 (Product # 88-7772-72), Anti-Mouse CD117 (c-Kit) APC (Product # 17-1171-82), and 0.125 µg of Anti-Mouse Ly-6A/E (Sca-1) PE. Lineage negative/low, viable cells, as determined by 7-AAD (Product # 00-6993-50), were used for analysis.

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参考文献：

1.Epigenetics and chromatinEstablishment of regulatory elements during erythro-megakaryopoiesis identifies hematopoietic lineage-commitment points.AuthorsHeuston EF,Keller CA,Lichtenberg J,Giardine B,Anderson SM,Hardison RC,Bodine DM2.Nature communicationsInduced p53 loss in mouse luminal cells causes clonal expansion and development of mammary tumours.3.Nature communicationsStem cell factor is selectively secreted by arterial endothelial cells in bone marrow.4.Stem cell reportsA Functional Bioluminescent Zebrafish Screen for Enhancing Hematopoietic Cell Homing.5.Nature immunologyThe transcriptional regulator Aire binds to and activates super-enhancers."12-5981 was used in Flow cytometry/Cell sorting to demonstrate the interaction between AIRE and partner proteins and long chromatin regions containing many super-enhancers."AuthorsBansal K,Yoshida H,Benoist C,Mathis D

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