CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), FITC, eBioscience™

货号：11-0495-82,11-0495-80

规格：100 µg,25 µg

价格：2243,957

产品类型：流式抗体

品牌：eBioscience

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eBioGoH3 (GoH3)

荧光染料：FITC

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™
浓度：	0.5 mg/mL	用法：	0.25 µg/test(Flow)

产品详细信息Description: The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA-6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex; CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells.CD49f expression has also been found on germinal center B cells. The eBioGoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin.Applications Reported: This GoH3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioGoH3 (GoH3) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CDf (Integrin alpha-6, ITGA6) is the integrin alpha chain alpha 6 protein. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signaling. Two transcript variants encoding different isoforms have been found for CDf. Functionally, integrins are cell surface receptors that interact with the extracellular matrix and mediate intracellular signals that affect cellular shape, mobility, and progression through the cell cycle in response to the extracellular matrix. Integrin receptors are composed of alpha and beta subunits, and form structural and functional linkages between the ECM (extra-cellular matrix) and intracellular cytoskeletal linker proteins. Signaling mediated by Intergrin/ECM interactions are also integrated with cellular responses to growth factor signaling to regulate cellular proliferation, cytoskeletal reorganization and other responses necessary for cellular survival. CDf is predominantly expressed by epithelia, and plays a critical structural role in the hemidesmosome. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells.

数据

CD49f (Integrin alpha 6) Antibody (11-0495-82) in FlowStaining of normal human peripheral blood cells with 0.125 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) (blue histogram) or 0.125 µg of Anti-Human/Mouse CD49f (Integrin alpha 6) FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Breast cancer research : BCRIntegrin-Rac signalling for mammary epithelial stem cell self-renewal.2.CellRegulatory T Cells in Skin Facilitate Epithelial Stem Cell Differentiation."11-0495 was used in Flow cytometry/Cell sorting to study the role of regulatory T cells in hair follicles and hair follicle stem cell biology."AuthorsAli N,Zirak B,Rodriguez RS,Pauli ML,Truong HA,Lai K,Ahn R,Corbin K,Lowe MM,Scharschmidt TC,Taravati K,Tan MR,Ricardo-Gonzalez RR,Nosbaum A,Bertolini M,Liao W,Nestle FO,Paus R,Cotsarelis G,Abbas AK,Rosenblum MD3.OncotargetLlgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration.4.OncotargetInhibition of the glucocorticoid receptor results in an enhanced miR-99a/100-mediated radiation response in stem-like cells from human prostate cancers.5.PloS oneMerlin Isoforms 1 and 2 Both Act as Tumour Suppressors and Are Required for Optimal Sperm Maturation.

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