Actin Monoclonal Antibody (mAbGEa)

货号：MA1-744

规格：100 µL

价格：5442

产品类型：免疫组化一抗

品牌：eBioscience

物种：人/小鼠/大鼠

宿主：大鼠

抗体亚型：IgG

荧光染料：Purified

类型:	单抗	同型对照：
浓度：		用法：	Assay dependent（ELISA）1:10-1:100(ICC);1:100(IF);1:100（IHC ）；1:10-1:1000(WB)
免疫原性：	Purified Arabidopsis actin protein.

产品详细信息MA1-744 detects actin protein in human, mouse, rat, bovine, sheep, drosophila, zebra fish (Danio rerio), yeast, arabidopsis and xenopus samples. This antibody has shown cross-reactivity with actin 1, 2, 3, 4, 7, 8, 11 and 12.MA1-744 has successfully been used in Western blot and ELISA procedures. By Western blot, this antibody detects a 45 kDa protein representing actin.The MA1-744 immunogen is purified actin protein from Arabidopsis.靶标信息Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

数据

Actin Antibody (MA1-744) in IHCImmunohistochemistry was performed on normal deparaffinized Human skeletal muscle tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Anti-Actin (Product # MA1-744) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. Actin Antibody (MA1-744) in IHCImmunohistochemistry was performed on normal deparaffinized Human tonsil tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Anti-Actin (Product # MA1-744) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. Actin Antibody (MA1-744) in IFImmunofluorescent analysis of Actin using Anti-Actin Monoclonal Antibody (mAbGEa) (Product # MA1-744) shows staining in A375 Cells. Actin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Actin (Product # MA1-744) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

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参考文献：

1.Communications biologyConserved Pbp1/Ataxin-2 regulates retrotransposon activity and connects polyglutamine expansion-driven protein aggregation to lifespan-controlling rDNA repeats.2.Veterinary researchMicroRNA expression profiling of goat peripheral blood mononuclear cells in response to peste des petits ruminants virus infection.3.Yeast (Chichester, England)Cost-effective and rapid lysis of Saccharomyces cerevisiae cells for quantitative western blot analysis of proteins, including phosphorylated eIF2α.4.PloS oneSubsets of Visceral Adipose Tissue Nuclei with Distinct Levels of 5-Hydroxymethylcytosine."MA1744 was used in western blot to test if adipose tissues have epigenetically distinct subpopulations of adipocytes"5.The Plant cellThe Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria.

技术参数

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