Exonuclease I (Exo I), the product of the sbcB gene of E. coli, is an exodeoxyribonuclease that hydrolyzes single-stranded DNA (ssDNA) stepwise in a 3′→5′ direction. Hydrolysis generates deoxyribonucleoside 5′ monophosphates and a terminal dinucleotide diphosphate.The enzyme requires magnesium (optimal Mg2+ concentration is 10 mM) and the presence of a free 3′-hydroxyl terminus.1 Exo I is active under a wide variety of buffer conditions, allowing addition of the enzyme directly into most reaction mixes. Heat inactivation results from incubation at 80°C for 15 minutes. Exo I is available in a 20,000-Unit size at a concentration of 20 Units/µL核酸外切酶Ⅰ以3’→5’方向消化ssDNA，但不消化dsDNA。虽然其活性依赖于镁离子和游离3’-OH末端存在，但它在各种缓冲液条件下都是有活性的，并且可以直接加入到大多数反应混合物中。在80℃温育15分钟导致热灭活。
优点：
- Specific: Processively digest ssDNA in a 3´ to 5´ direction without harming dsDNA - short 3´ overhangs in DNA are not digested；- Flexible: Active under a wide variety of buffer conditions and can be added directly into most reaction mixes；- Heat Inactivated: Destroy the enzyme activity by incubating at 80°C for 15 minutes
相关产品：
▪2.5 mM dNTP Mix（#30030）
参考文献：
1. Lehman, I.R. and Nussbaum, A.L. (1964) J. Biol. Chem. 239, 2628. 2. Kusher, S.R. et al., (1971) Proc. Natl. Acad. Sci. USA 68, 824. 3. Kusher, S.R. et al., (1972) Proc. Natl. Acad. Sci. USA 69, 1366. 4. Goldmark, P.J. and Linn, S. (1972) J. Biol. Chem. 247, 1849. 5. Rosamond, J. et al., (1979) J. Biol. Chem. 254, 8646.
技术参数
产品优点 - Specific: Processively digest ssDNA in a 3´ to 5´ direction without harming dsDNA - short 3´ overhangs in DNA are not digested
- Flexible: Active under a wide variety of buffer conditions and can be added directly into most reaction mixes
- Heat Inactivated: Destroy the enzyme activity by incubating at 80°C for 15 minutes
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