CloneSmart HCKan Blunt Cloning Kit,CloneSmart HCKan平末端克隆试剂盒
货号:40704-2,40704-4
规格:20 rxns ,40 rxns
价格:3505,5735
产品类型:二代测序-建库
品牌:Lucigen
Lucigen’s unique cloning kits with pre-processed vectors and highly competent cells eliminate hours of tedious preparation and enable efficient cloning. Ligation can be completed in as little as 30 minutes with no clean-up needed; only a brief heat denaturation is necessary before transformation. Downstream manipulation (e.g, mutagenesis, subsequent insert addition) is facilitated by the minimal vector size (1.7-2.0 kb). |
优点: |
▪ Convenient, pre-processed vectors eliminate the need for digestion, gel purification, and dephosphorylation;▪ Transcription terminators flanking cloning site stabilize otherwise toxic inserts;▪ Choice of copy number and antibiotic resistance. |
数据: |
Figure 1.High copy (HC) and low copy (LC) versions of the pSMART transcription-free cloning vectors. |
相关产品: |
▪CloneSmart HCAmp Blunt Cloning Kit(货号#40041)▪ CloneSmart LCAmp Blunt Cloning Kit(货号#40300)▪CloneSmart LCKan Blunt Cloning Kit(货号#40821) |
技术参数 产品优点 - Convenient, pre-processed vectors eliminate the need for digestion, gel purification, and dephosphorylation. - Transcription terminators flanking cloning site stabilize otherwise toxic inserts. - Choice of copy number and antibiotic resistance.
组成成分 - 4X CloneSmart Vector Premix Includes Buffer, ATP, and one type of ligation-ready Vector - CloneSmart DNA Ligase (2 U/µL) - Positive Control Insert DNA (500 ng/µL lambda HincII) - CloneSmart Sequencing Primers (200 reactions each) SL1 Primer (3.2 pmol/µL) SR2 Primer (3.2 pmol/µL)