MaxPlax™ Lambda Packaging Extracts（停产）
货号：MP5105,MP5110,MP5120
规格：5 extracts,10 extracts,20 extracts
价格：2691,4345,7084
产品类型：质粒
品牌：Lucigen
| MaxPlax™ Lambda Packaging Extracts are a convenient, high-efficiency transduction system designed for in vitro lambda packaging reactions. MaxPlax Lambda Packaging Extracts are supplied as predispensed single-tube reactions that have been optimized for packaging of methylated and unmethylated DNA.The packaging extracts routinely yield packaging efficiencies of >1 × 109 pfu/μg of Ligated Lambda Control DNA. The extracts can be used in the construction of representative cDNA libraries and genomic cloning of highly modified (methylated) DNA into λ-phage or cosmid vectors.Traditional packaging extracts are derived from two complementary lysogenic E. coli strains, BHB2690 and BHB2688, as described by Hohn (1979).The MaxPlax extracts utilize a new packaging strain, NM759, reported by Gunther, Murray and Glazer (1993).This strain, which replaces strain BHB2690 in the preparation of the sonication extract, is a restrictionfree K12-derived strain deficient in the production of λ-phage capsid protein D. When combined with the complementary freeze-thaw extract from strain BHB2688,deficient in the production of λ-phage capsid protein E, an extremely high-efficiency of packaging for λDNA is obtained. Moreover, the ability to package λDNA bearing the mammalian methylation pattern is greatly enhanced, as evidenced by the high efficiency of λ-vector rescue from transgenic mouse DNA.The lack of restriction activity has been shown to be crucial for the high efficiency rescue of lambda shuttle vectors from transgenic mouse DNA. |
| 优点： |
| ▪ Construct genomic libraries from methylated or unmethylated DNA with equal efficiency due to the lack of all known restriction activities in the extracts.▪ Packaging efficiencies of up to 3 × 109 pfu/µg DNA.▪ Simplified protocol does not require premixing of different extract components before use |
| 组成成分： |
| ▪ MaxPlax Lambda Packaging Extract：储存在-70℃▪LE392MP Control Plating Strain Glycerol Stock：储存在-70℃▪ Ligated Lambda Control DNA (0.02 µg/µL)：储存在-70℃ |
| 参考文献： |
| 1. Hohn, E.G. (1979) Methods Enzymol. 68, 299. 2. Gunther, E.G. et al., (1993) Nucl. Acids Res. 21, 3903. 3. Kohler, S.W. et al., (1990) Nucl. Acids Res. 18, 3007. |
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产品优点 - Construct genomic libraries from methylated or unmethylated DNA with equal efficiency due to the lack of all known restriction activities in the extracts.
- Packaging efficiencies of up to 3 × 109 pfu/µg DNA.
- Simplified protocol does not require premixing of different extract components before use
产品应用 - The extracts can be used in the construction of representative cDNA libraries and genomic cloning of highly modified (methylated) DNA into λ-phage or cosmid vectors.
QQ:1749072012