HI-Control™ 10G Chemically Competent Cells (SOLOs)/HI-Control™10G化学感受态细胞
货号：60110-1
规格： 12 rxns (SOLOs)
价格：2646
产品类型：感受态细胞
品牌：Lucigen
| HI-Control 10G cells are an E. coli strain optimized for high efficiency transformation. They are ideal for
cloning and propagation of plasmid clones. They give high yield and high quality plasmid DNA due to the
endA1 mutation. HI-Control 10G cells harbor a single-copy BAC plasmid carrying an engineered lacIq1
repressor allele.The lacIq1 allele expresses approximately 170-fold more Lac repressor than does the wildtype
lacI gene.The HI-Control 10G strain does not contain T7 RNA polymerase, and the excess lac
repressor in this strain further minimizes any background transcription by the bacterial polymerase. HI-Control 10G细胞是为高效转化而优化的大肠杆菌菌株。 它们是克隆和繁殖质粒克隆的理想选择。 由于endA1突变，它们可提供高产量和高质量的质粒DNA。 HI-Control 10G细胞带有携带工程化lacIq1阻遏物等位基因的单拷贝BAC质粒.lacIq1等位基因表达的Lac阻遏物约比野生型lacI基因多170倍.HI-Control 10G菌株不含T7 RNA聚合酶， 并且该菌株中过量的lac阻遏物进一步减少了细菌聚合酶的任何背景转录。 |
| 优点： |
| ▪ Inducehigh level protein expressionfromlacbased promoters (T7, T5-lac, tac, trc,lac)；▪ Tightly regulateexpression from T7 promoters with HI-Control BL21(DE3) Chemically Competent Cells；▪ Clone targetsandreduce background protein expression with HI-Control 10G Chemically Competent Cells ▪ 从基于lac的启动子（T7，T5-lac，tac，trc，lac）诱导高水平的蛋白表达▪ 用HI-Control BL21（DE3）化学感受态细胞严格调节T7启动子的表达▪ 利用HI-Control 10G化学感受态细胞克隆靶标并降低背景蛋白的表达 |
| 相关产品： |
| ▪HI-Control™ BL21(DE3) Chemically Competent Cells (SOLOs)（货号#60435） |
技术参数 产品优点 - Induce high level protein expression from lac based promoters (T7, T5-lac, tac, trc, lac) - Tightly regulate expression from T7 promoters with HI-Control BL21(DE3) Chemically Competent Cells - Clone targets and reduce background protein expression with HI-Control 10G Chemically Competent Cells- 从基于lac的启动子（T7，T5-lac，tac，trc，lac）诱导高水平的蛋白表达- 用HI-Control BL21（DE3）化学感受态细胞严格调节T7启动子的表达- 利用HI-Control 10G化学感受态细胞克隆靶标并降低背景蛋白的表达
组成成分 Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
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