BAC-Optimized Replicator v2.0 Electrocompetent Cells，BAC优化的Replicator v2.0电感受态细胞
货号：60210-1 ,60210-2
规格：12 rxns (DUOs) ,24 rxns (DUOs)
价格：2467,4270
产品类型：感受态细胞
品牌：Lucigen
| Lucigen’s BAC-Optimized Replicator v2.0 Electrocompetent Cells are an E. coli strain that contains an
inducible trfA gene, which is required for induction of replication from the oriV origin in BAC and fosmid
clones. Most lab strains of E. coli do not contain a trfA gene, and thus will not support copy number
amplification of such vectors.
BAC-Optimized Replicator v2.0 Cells are designed for high efficiency transformation by electroporation,
yielding >1 x 1010 cfu/µg pKanR DNA. These cells are ideal for cloning and propagating BAC, fosmid or
plasmid clones. In addition to the trfA gene, they contain the endA1 mutation for high yield and high
quality plasmid DNA. They also contain the mcr and mrr mutations, stable cloning of methylated
genomic DNA isolated directly from mammalian or plant cells. They do not contain the F plasmid. The
rpsL mutation confers resistance to streptomycin. |
| 优点： |
| ▪Cells that enable the most challenging library construction and screening；▪Highest available transformation efficiencies for library construction and screening；▪Optimized for high yields of DNA； |
| 相关产品： |
| ▪Recovery Medium（货号#80026-1）▪ E. cloni 10G BAC-Optimized Electrocompetent Cells（货号#60215-2） |
产品优点 - Cells that enable the most challenging library construction and screening. - Highest available transformation efficiencies for library construction and screening. - Optimized for high yields of DNA.
产品应用 - These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimized Replicator Cells are components of Lucigen’s CopyRight® Cloning Kits.
组成成分 - Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube) or FIVERs (5 transformations per tube), as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
QQ:1749072012