Exo-Minus Klenow DNA Poly (D355A, E357A)
货号：KL11101K
规格：1,000 U @ 10 U/µL
价格：3557
产品类型：RNA-Seq建库
品牌：Lucigen
| Exo-Minus Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both of the 5′→3′ and 3′→5′ exonuclease activities of E. coli DNA Polymerase I from which it is derived. This N-terminal truncation of DNA Polymerase I has two mutations (D355A and E357A). Exo-Minus Klenow DNA聚合酶是一种DNA依赖性DNA聚合酶，缺乏其所来源的大肠杆菌DNA聚合酶Ⅰ的5’→3’和3’→5’核酸外切酶活性。N-末端截断的DNA聚合酶Ⅰ也有两个突变（D355A和E357A）。 |
| 优点： |
| ▪ Synthesize DNA from ssDNA templates without degrading any single-stranded DNA▪ Suitable for a variety of applications including random-primer labeling of DNA,1second-strand cDNA synthesis,2sStrand-displacement amplification3 |
| 组成成分： |
| ▪ Exo-Minus Klenow DNA Polymerase (D355A, E357A) (10 U/µL):储存在-20℃▪ Klenow exo- DNA Pol. 10X Buffer:储存在-20℃ |
| 参考文献： |
| 1. Lehman, I.R. (1981) The Enzymes Academic Press 14, 16. |
技术参数 产品优点 - Synthesize DNA from ssDNA templates without degrading any single-stranded DNA
- Suitable for a variety of applications including random-primer labeling of DNA,1second-strand cDNA synthesis,2sStrand-displacement amplification3
产品应用 - Random-primer labeling of DNA,1second-strand cDNA synthesis,2sStrand-displacement amplification3
- DNA的随机引物标记
- 第二链cDNA合成
- 链置换扩增
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